ICAD Antibody (F-8): sc-17816

ICAD Antibody (F-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects ICAD protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ICAD (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ICAD protein, also known as DFF-45, plays a crucial role in the apoptotic process by acting as an inhibitor of the DNA fragmentation factor, which is essential for the controlled dismantling of cellular components during apoptosis. When cleaved by caspases, particularly CPP32, ICAD releases its inhibitory effect, allowing the activation of the DNA fragmentation factor that leads to the fragmentation of nuclear DNA, a hallmark of apoptosis. This process is vital for maintaining tissue homeostasis and preventing the development of cancer, as ICAD ensures damaged or unnecessary cells are efficiently removed from the organism. ICAD′s ability to regulate apoptosis highlights its importance in both normal physiological processes and in the pathogenesis of various diseases, making anti-ICAD antibody (F-8) a valuable tool for researchers studying cell death and its implications in health and disease.

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ICAD Antibody (F-8) References:

1. Differential localization of ICAD-L and ICAD-S in cells due to removal of a C-terminal NLS from ICAD-L by alternative splicing.  |  Samejima, K. and Earnshaw, WC. 2000. Exp Cell Res. 255: 314-20. PMID: 10694446
2. Endovascular treatment for AIS with underlying ICAD.  |  Al Kasab, S., et al. 2017. J Neurointerv Surg. 9: 948-951. PMID: 27502403
3. Submaximal angioplasty in the treatment of patients with symptomatic ICAD: a systematic review and meta-analysis.  |  Seyedsaadat, SM., et al. 2020. J Neurointerv Surg. 12: 380-385. PMID: 31748381
4. Association of Plaque Morphology With Stroke Mechanism in Patients With Symptomatic Posterior Circulation ICAD.  |  Song, X., et al. 2022. Neurology. 99: e2708-e2717. PMID: 36220598
5. Human plasma gelsolin binds to fibronectin.  |  Lind, SE. and Janmey, PA. 1984. J Biol Chem. 259: 13262-6. PMID: 6092370
6. Mch3, a novel human apoptotic cysteine protease highly related to CPP32.  |  Fernandes-Alnemri, T., et al. 1995. Cancer Res. 55: 6045-52. PMID: 8521391
7. Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis.  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
8. Lamin proteolysis facilitates nuclear events during apoptosis.  |  Rao, L., et al. 1996. J Cell Biol. 135: 1441-55. PMID: 8978814
9. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis.  |  Liu, X., et al. 1997. Cell. 89: 175-84. PMID: 9108473
10. Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis.  |  Kothakota, S., et al. 1997. Science. 278: 294-8. PMID: 9323209
11. Caspases: intracellular signaling by proteolysis.  |  Salvesen, GS. and Dixit, VM. 1997. Cell. 91: 443-6. PMID: 9390553
12. ICAD/DFF regulator of apoptotic nuclease is nuclear.  |  Samejima, K. and Earnshaw, WC. 1998. Exp Cell Res. 243: 453-9. PMID: 9743604

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ICAD Antibody (F-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ICAD Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ICAD Antibody (F-8) Specifications

Alternate Product Name: ICAD

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-331

Epitope Target: ICAD

Epitope Species: human

Product Reactivity/Detection: ICAD

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ICAD Antibody (F-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin