HtrA2 Antibody (E-3): sc-365594

HtrA2 Antibody (E-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects HtrA2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-HtrA2 antibody (E-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HtrA2, also known as Omi, is a member of the serine protease family and plays a crucial role in cellular stress responses by degrading misfolded proteins, thereby preventing the accumulation of potentially toxic aggregates. HtrA2 is predominantly localized in the mitochondria and is involved in regulating apoptosis, making HtrA2 essential for maintaining cellular homeostasis. HtrA2 is expressed in various tissues, including the kidney, colon, and thyroid, and HtrA2 expression is tightly regulated; alterations in HtrA2 levels have been implicated in several diseases, including neurodegenerative disorders and cancer. HtrA2 exists in two polypeptide forms and features a modified PDZ domain that influences HtrA2 interactions with other proteins, such as the Mxi2 protein, which is not recognized by the alternatively spliced D-Omi form. The conservation of the HtrA2 gene across mammalian species underscores HtrA2′s fundamental biological importance, and HtrA2′s ability to undergo autoproteolysis further highlights HtrA2′s dynamic role in cellular processes.

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HtrA2 Antibody (E-3) References:

1. Expression, purification, and functional analysis of the human serine protease HtrA2.  |  Savopoulos, JW., et al. 2000. Protein Expr Purif. 19: 227-34. PMID: 10873535
2. Characterization of human HtrA2, a novel serine protease involved in the mammalian cellular stress response.  |  Gray, CW., et al. 2000. Eur J Biochem. 267: 5699-710. PMID: 10971580
3. Tissue-specific splicing of Omi stress-regulated endoprotease leads to an inactive protease with a modified PDZ motif.  |  Faccio, L., et al. 2000. Genomics. 68: 343-7. PMID: 10995577
4. Temperature dependent protease activity and structural properties of human HtrA2 protease.  |  Zhang, X. and Chang, Z. 2004. Biochemistry (Mosc). 69: 687-92. PMID: 15236609
5. Immunohistochemical analysis of Omi/HtrA2 expression in prostate cancer and benign prostatic hyperplasia.  |  Hu, XY., et al. 2006. APMIS. 114: 893-8. PMID: 17207090
6. HtrA2 regulates beta-amyloid precursor protein (APP) metabolism through endoplasmic reticulum-associated degradation.  |  Huttunen, HJ., et al. 2007. J Biol Chem. 282: 28285-95. PMID: 17684015
7. Omi/HtrA2 and XIAP are components of platelet apoptosis signalling.  |  Winkler, J., et al. 2013. Thromb Haemost. 109: 532-9. PMID: 23306356
8. Stress Conditions Increase Vimentin Cleavage by Omi/HtrA2 Protease in Human Primary Neurons and Differentiated Neuroblastoma Cells.  |  Lucotte, B., et al. 2015. Mol Neurobiol. 52: 1077-1092. PMID: 25288153
9. Inhibition of HtrA2 alleviated dextran sulfate sodium (DSS)-induced colitis by preventing necroptosis of intestinal epithelial cells.  |  Zhang, C., et al. 2019. Cell Death Dis. 10: 344. PMID: 31019191
10. Serine Protease HtrA2/Omi Deficiency Impairs Mitochondrial Homeostasis and Promotes Hepatic Fibrogenesis via Activation of Hepatic Stellate Cells.  |  Hur, W., et al. 2019. Cells. 8: PMID: 31547195
11. Primary structure of a putative serine protease specific for IGF-binding proteins.  |  Zumbrunn, J. and Trueb, B. 1996. FEBS Lett. 398: 187-92. PMID: 8977104
12. Human HtrA, an evolutionarily conserved serine protease identified as a differentially expressed gene product in osteoarthritic cartilage.  |  Hu, SI., et al. 1998. J Biol Chem. 273: 34406-12. PMID: 9852107

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HtrA2 Antibody (E-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HtrA2 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HtrA2 Antibody (E-3) Specifications

Alternate Product Name: HtrA2

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 341-400

Epitope Target: HtrA2

Epitope Species: human

Product Reactivity/Detection: HtrA2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HtrA2 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin