HSP 90α Antibody (F-2): sc-515081

HSP 90α Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSP 90 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-HSP 90α antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSP 90 alpha protein plays a crucial role as a molecular chaperone, assisting in proper folding and stabilization of numerous client proteins, including steroid hormone receptors and kinases, which are vital for cellular signaling and stress responses. This chaperone activity is particularly important in maintaining cellular homeostasis under stress conditions, such as heat shock or oxidative stress, where HSP 90 alpha helps prevent aggregation and misfolding of other proteins. Additionally, HSP 90 alpha forms complexes with various co-chaperones, such as p60/stip1 and p23, which further regulate activity and client protein interactions. HSP 90 alpha′s ability to modulate key signaling proteins underscores significance in cancer biology, as many oncogenic proteins depend on HSP 90 alpha for stability and function, making this protein a potential target for therapeutic intervention.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HSP 90α Antibody (F-2) References:

1. PKC epsilon is a unique regulator for hsp90 beta gene in heat shock response.  |  Wu, JM., et al. 2003. J Biol Chem. 278: 51143-9. PMID: 14532285
2. HSP90 and the chaperoning of cancer.  |  Whitesell, L. and Lindquist, SL. 2005. Nat Rev Cancer. 5: 761-72. PMID: 16175177
3. Hsp90 potentiates the rapid evolution of new traits: drug resistance in diverse fungi.  |  Cowen, LE. and Lindquist, S. 2005. Science. 309: 2185-9. PMID: 16195452
4. Modulating molecular chaperone Hsp90 functions through reversible acetylation.  |  Aoyagi, S. and Archer, TK. 2005. Trends Cell Biol. 15: 565-7. PMID: 16199163
5. The HSP90 family of genes in the human genome: insights into their divergence and evolution.  |  Chen, B., et al. 2005. Genomics. 86: 627-37. PMID: 16269234
6. Hsp90: a chaperone for protein folding and gene regulation.  |  Zhao, R. and Houry, WA. 2005. Biochem Cell Biol. 83: 703-10. PMID: 16333321
7. Hsp90 regulates activation of interferon regulatory factor 3 and TBK-1 stabilization in Sendai virus-infected cells.  |  Yang, K., et al. 2006. Mol Biol Cell. 17: 1461-71. PMID: 16394098
8. Substrate transfer from the chaperone Hsp70 to Hsp90.  |  Wegele, H., et al. 2006. J Mol Biol. 356: 802-11. PMID: 16403523
9. Modulation of chaperone function and cochaperone interaction by novobiocin in the C-terminal domain of Hsp90: evidence that coumarin antibiotics disrupt Hsp90 dimerization.  |  Allan, RK., et al. 2006. J Biol Chem. 281: 7161-71. PMID: 16421106

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSP 90α Antibody (F-2) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-HSP 90α Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSP 90α Antibody (F-2) Specifications

Alternate Product Name: HSP90

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 342-382

Epitope Target: HSP 90α

Epitope Species: human

Product Reactivity/Detection: HSP 90α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for HSP 90α Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HSP90 alpha/beta antibody is HSP90 alpha/beta Antibody (F-8): sc-13119.