HSP 75 Antibody (TR1): sc-13557

HSP 75 Antibody (TR1) is a mouse monoclonal IgG2a kappa light chain antibody that detects HSP 75 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HSP 75 antibody (TR1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heat shock protein 75, also known as tumor necrosis factor type 1 receptor-associated protein (TRAP1), is a crucial mitochondrial chaperone composed of 704 amino acids and belongs to the heat shock protein 90 family. HSP 75 assists in proper protein folding and assembly within mitochondria, which is vital for maintaining mitochondrial function and cellular homeostasis. Disruptions in HSP 75 function can lead to mitochondrial dysfunction, contributing to various diseases, including neurodegenerative disorders and cancer. HSP 75 localization in mitochondria underscores its importance in energy metabolism and apoptosis regulation, making HSP 75 a significant target for research in cellular stress responses and therapeutic interventions.

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HSP 75 Antibody (TR1) References:

1. Stress protein response in two sibling species of Marenzelleria (Polychaeta: Spionidae): is there an influence of acclimation salinity?  |  Blank, M., et al. 2006. Comp Biochem Physiol B Biochem Mol Biol. 144: 451-62. PMID: 16807029
2. Iron chelation study in a normal human hepatocyte cell line suggests that tumor necrosis factor receptor-associated protein 1 (TRAP1) regulates production of reactive oxygen species.  |  Im, CN., et al. 2007. J Cell Biochem. 100: 474-86. PMID: 16927372
3. Identification of the divergent calmodulin binding motif in yeast Ssb1/Hsp75 protein and in other HSP70 family members.  |  Heinen, RC., et al. 2006. Braz J Med Biol Res. 39: 1399-408. PMID: 17146552
4. Varying responses of human cells with discrepant p53 activity to ionizing radiation and heat shock exposure.  |  Tokalov, SV., et al. 2007. Cell Prolif. 40: 24-37. PMID: 17227293
5. Identification of endosomal epidermal growth factor receptor signaling targets by functional organelle proteomics.  |  Stasyk, T., et al. 2007. Mol Cell Proteomics. 6: 908-22. PMID: 17293594
6. Heat shock protein 75 (TRAP1) antagonizes reactive oxygen species generation and protects cells from granzyme M-mediated apoptosis.  |  Hua, G., et al. 2007. J Biol Chem. 282: 20553-60. PMID: 17513296
7. The Mitochondrial Chaperone TRAP1 as a Candidate Target of Oncotherapy.  |  Xie, S., et al. 2020. Front Oncol. 10: 585047. PMID: 33575209
8. TRAP1 in Oxidative Stress and Neurodegeneration.  |  Ramos Rego, I., et al. 2021. Antioxidants (Basel). 10: PMID: 34829705
9. TRAP1 Chaperones the Metabolic Switch in Cancer.  |  Wengert, LA., et al. 2022. Biomolecules. 12: PMID: 35740911
10. Targeting the Mitochondrial Chaperone TRAP1 Alleviates Vascular Pathologies in Ischemic Retinopathy.  |  Kim, SY., et al. 2024. Adv Sci (Weinh). 11: e2302776. PMID: 37983591
11. TRAP1 drives smooth muscle cell senescence and promotes atherosclerosis via HDAC3-primed histone H4 lysine 12 lactylation.  |  Li, X., et al. 2024. Eur Heart J. 45: 4219-4235. PMID: 39088352
12. TRAP1 modulates mitochondrial biogenesis via PGC-1α/TFAM signalling pathway in colorectal cancer cells.  |  Bruno, G., et al. 2024. J Mol Med (Berl). 102: 1285-1296. PMID: 39210159

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSP 75 Antibody (TR1) has 28 citations in a variety of scientific publications.

Primary antibodies like Anti-HSP 75 Antibody (TR1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSP 75 Antibody (TR1) Specifications

Alternate Product Name: HSP 75

Product Clone Name: TR1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: HSP 75

Epitope Species: human

Product Reactivity/Detection: HSP 75

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 28 citations in various scientific publications

Available Conjugates/Forms for HSP 75 Antibody (TR1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin