HSP 47 Antibody (G-12): sc-5293

HSP 47 Antibody (G-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects HSP 47 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-HSP 47 antibody (G-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSP 47, also known as collagen 1, serpinh1, and collagen-binding protein 1 (CBP1), is primarily located in the endoplasmic reticulum of procollagen-producing cells, where HSP 47 plays a crucial role in proper folding and assembly of collagen. HSP 47 is essential for biosynthesis of Collagen Type I, which maintains structural integrity of various tissues, including skin, bone, and cartilage. Through molecular chaperone activity, HSP 47 ensures procollagen folds correctly before secretion from cells, preventing accumulation of misfolded proteins that could lead to cellular stress and tissue damage. HSP 47 synthesis increases in response to transforming growth factor beta (TGFβ) and interleukin-1 beta (IL-1β), highlighting HSP 47′s role in cellular stress responses and tissue remodeling.

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HSP 47 Antibody (G-12) References:

1. Biology of Hsp47 (Serpin H1), a collagen-specific molecular chaperone.  |  Ito, S. and Nagata, K. 2017. Semin Cell Dev Biol. 62: 142-151. PMID: 27838364
2. TFE3, HSP47, and CREB3 Pathways of the Mammalian Golgi Stress Response.  |  Taniguchi, M. and Yoshida, H. 2017. Cell Struct Funct. 42: 27-36. PMID: 28179603
3. The chaperone protein HSP47: a platelet collagen binding protein that contributes to thrombosis and hemostasis.  |  Sasikumar, P., et al. 2018. J Thromb Haemost. 16: 946-959. PMID: 29512284
4. Roles of the endoplasmic reticulum-resident, collagen-specific molecular chaperone Hsp47 in vertebrate cells and human disease.  |  Ito, S. and Nagata, K. 2019. J Biol Chem. 294: 2133-2141. PMID: 30541925
5. Hsp47 promotes cancer metastasis by enhancing collagen-dependent cancer cell-platelet interaction.  |  Xiong, G., et al. 2020. Proc Natl Acad Sci U S A. 117: 3748-3758. PMID: 32015106
6. Effect of Regulating the Expression of HSP47 on Collagen Metabolism in Scleral Fibroblasts.  |  Guo, Q., et al. 2021. Curr Eye Res. 46: 408-416. PMID: 32814454
7. HSP47 contributes to angiogenesis by induction of CCL2 in bladder cancer.  |  Ma, W., et al. 2021. Cell Signal. 85: 110044. PMID: 34000383
8. Targeting HSP47 and HSP70: promising therapeutic approaches in liver fibrosis management.  |  Abd El-Fattah, EE. and Zakaria, AY. 2022. J Transl Med. 20: 544. PMID: 36435779
9. HSP47 Increases the Expression of Type I Collagen in Fibroblasts through IRE1α Activation, XBP1 Splicing, and Nuclear Translocation of β-Catenin.  |  Ham, SY., et al. 2024. Cells. 13: PMID: 38534372
10. Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum.  |  Wang, YJ., et al. 2024. Elife. 13: PMID: 38963323

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSP 47 Antibody (G-12) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-HSP 47 Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSP 47 Antibody (G-12) Specifications

Alternate Product Name: SERPINH1

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 129-300

Epitope Target: HSP 47

Epitope Species: human

Product Reactivity/Detection: HSP 47

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for HSP 47 Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HSP 47 antibody is HSP 47 Antibody (E-1): sc-13150.