HSP 47 Antibody (E-1): sc-13150

HSP 47 Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSP 47 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HSP 47 antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSP 47, also known as collagen 1, serpinh1, collagen-binding protein 1 (CBP1), and gp46, is predominantly located in the endoplasmic reticulum of procollagen-producing cells, where HSP 47 plays a crucial role in proper folding and assembly of collagen. HSP 47 is essential for biosynthesis of Collagen Type I, which maintains structural integrity of various tissues. As a molecular chaperone, HSP 47 ensures procollagen is correctly processed and secreted, preventing accumulation of misfolded proteins that could lead to cellular stress and tissue damage. HSP 47 synthesis increases in response to growth factors such as TGFβ and pro-inflammatory cytokines like IL-1β, highlighting HSP 47′s importance in both normal physiological processes and stress responses.

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HSP 47 Antibody (E-1) References:

1. Co-expression of colligin and collagen in oral submucous fibrosis: plausible role in pathogenesis.  |  Kaur, J., et al. 2001. Oral Oncol. 37: 282-7. PMID: 11287283
2. Distribution of heat shock proteins in kidneys of rats after immunosuppressive treatment with cyclosporine A.  |  Stacchiotti, A., et al. 2001. Acta Histochem. 103: 167-77. PMID: 11368098
3. Heat shock protein (HSP) 47 and collagen are upregulated during neointimal formation in the balloon-injured rat carotid artery.  |  Murakami, S., et al. 2001. Atherosclerosis. 157: 361-8. PMID: 11472735
4. In vivo expression of the collagen-related heat shock protein HSP47, following hyperthermia or photodynamic therapy.  |  Verrico, AK., et al. 2001. Lasers Med Sci. 16: 192-8. PMID: 11482817
5. Involvement of heat shock proteins in the healing of acetic acid-induced gastric ulcers in rats.  |  Tsukimi, Y., et al. 2001. J Physiol Pharmacol. 52: 391-406. PMID: 11596858
6. Xaa-Arg-Gly triplets in the collagen triple helix are dominant binding sites for the molecular chaperone HSP47.  |  Koide, T., et al. 2002. J Biol Chem. 277: 6178-82. PMID: 11751879
7. Expression of heat shock proteins in a linear rodent wound.  |  Keagle, JN., et al. 2001. Wound Repair Regen. 9: 378-85. PMID: 11896981
8. Induction of heat shock protein 47 synthesis by TGF-beta and IL-1 beta via enhancement of the heat shock element binding activity of heat shock transcription factor 1.  |  Sasaki, H., et al. 2002. J Immunol. 168: 5178-83. PMID: 11994473
9. HSP47 levels determine the degree of body adiposity.  |  Shin, J., et al. 2023. Nat Commun. 14: 7319. PMID: 37951979
10. Targeting HSP47 for cancer treatment.  |  Shi, R., et al. 2024. Anticancer Drugs. 35: 623-637. PMID: 38718070
11. Targeting the HSP47-collagen axis inhibits brain metastasis by reversing M2 microglial polarization and restoring anti-tumor immunity.  |  Wang, L., et al. 2024. Cell Rep Med. 5: 101533. PMID: 38744278
12. HSP47 in human diseases: Navigating pathophysiology, diagnosis and therapy.  |  Khan, ES. and Däinghaus, T. 2024. Clin Transl Med. 14: e1755. PMID: 39135385

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSP 47 Antibody (E-1) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-HSP 47 Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSP 47 Antibody (E-1) Specifications

Alternate Product Name: SERPINH1

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 129-300

Epitope Target: HSP 47

Epitope Species: human

Product Reactivity/Detection: HSP 47

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for HSP 47 Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin