HSP 27 Antibody (G3.1) recommended for detection of HSP 27 of mouse, rat and human origin by WB, IP, IF and IHC(P)

HSP 27 Antibody (G3.1): sc-59562

HSP 27 Antibody (G3.1) is rated 4.3 out of 5 by 4.
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Datasheets

    • HSP 27 Antibody (G3.1) is a mouse monoclonal IgG1, cited in 5 publications, provided at 100 µg/ml
    • raised against partially purified HSP 27 derived from MCF-7 cytosol of human origin
    • recommended for detection of HSP 27 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See HSP 27 (F-4): sc-13132 for HSP 27 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can sc-59562: HSP 27 (G3.1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, sc-59562: HSP 27 (G3.1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from strong band Single strong band in WB(1/200) in mouse spleen tissue extract, very good.
Date published: 2017-05-13
Rated 4 out of 5 by from Recognized antigen in all transfeced lysates Recognized antigen in all transfeced lysates tested. -SCBT QC
Date published: 2015-04-12
Rated 4 out of 5 by from Publishable WB results in human primary epithelial Publishable WB results in human primary epithelial cells. -SCBT Publication Review
Date published: 2015-02-19
Rated 4 out of 5 by from Strong bands showing HSP 27 expression in Strong bands showing HSP 27 expression in HeLa and heat shock treated HeLa whole cell lysates. -SCBT QC
Date published: 2015-01-12
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