hnRNP I Antibody (SH54): sc-56701

hnRNP I Antibody (SH54) is a mouse monoclonal IgG1 kappa light chain antibody that detects hnRNP I protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-hnRNP I antibody (SH54) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heterogeneous nuclear ribonucleoprotein I (hnRNP I), also known as polypyrimidine tract-binding protein (PTB), plays a crucial role in regulating alternative splicing of pre-mRNAs, which is essential for generating protein diversity and regulating gene expression. hnRNP I is primarily localized in the nucleoplasm, where binding to the 3′ end of introns influences splicing mechanisms in normal cellular processes and translation of certain viruses, such as hepatitis C virus (HCV). The hnRNP I gene is located on chromosome 19p13.3, and interaction with other hnRNP proteins, like hnRNP L, underscores importance in the spliceosome′s composition, which is vital for mRNA processing. hnRNP I′s ability to shuttle between the nucleus and cytoplasm further highlights significance in mRNA transport and translation, making this protein a key player in cellular function and viral pathogenesis.

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hnRNP I Antibody (SH54) References:

1. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs.  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
2. Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm.  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
3. Organization of the human gene encoding heterogeneous nuclear ribonucleoprotein type I (hnRNP I) and characterization of hnRNP I related pseudogene.  |  Romanelli, MG., et al. 2000. Gene. 255: 267-72. PMID: 11024286
4. Binding of hnRNP I-vRNA Regulates Sindbis Virus Structural Protein Expression to Promote Particle Infectivity.  |  Westcott, CE., et al. 2022. Viruses. 14: PMID: 35891402
5. Increasing dietary nutrient levels modulates colon immune adaptation and alleviates inflammation in the epithelial heterogeneous nuclear ribonucleoprotein I (Hnrnp I) knockout mice.  |  Xu, GB., et al. 2023. J Nutr Biochem. 119: 109406. PMID: 37394079
6. Single-Cell RNA Sequencing (scRNA-seq) Identifies L1CAM as a Key Mediator between Epithelial Tuft Cell and Innate Lymphoid Cell in the Colon of Hnrnp I Knockout Mice.  |  Xu, GB., et al. 2023. Biomedicines. 11: PMID: 37893107
7. A nuclear localization domain in the hnRNP A1 protein.  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
8. Identification and characterisation of a novel human RNA-binding protein.  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
9. Multiple RRMs contribute to RNA binding specificity and affinity for polypyrimidine tract binding protein.  |  Pérez, I., et al. 1997. Biochemistry. 36: 11881-90. PMID: 9305981
10. Polypyrimidine tract-binding protein interacts with HnRNP L.  |  Hahm, B., et al. 1998. FEBS Lett. 425: 401-6. PMID: 9563502
11. Heterogeneous nuclear ribonucleoprotein L interacts with the 3' border of the internal ribosomal entry site of hepatitis C virus.  |  Hahm, B., et al. 1998. J Virol. 72: 8782-8. PMID: 9765422
12. Polypyrimidine tract-binding protein binds to the leader RNA of mouse hepatitis virus and serves as a regulator of viral transcription.  |  Li, HP., et al. 1999. J Virol. 73: 772-7. PMID: 9847386

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hnRNP I Antibody (SH54) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-hnRNP I Antibody (SH54) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hnRNP I Antibody (SH54) Specifications

Alternate Product Name: PTBP1

Product Clone Name: SH54

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: hnRNP I

Epitope Species: human

Product Reactivity/Detection: hnRNP I

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for hnRNP I Antibody (SH54): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred hnRNP I antibody is hnRNP I Antibody (A-4): sc-515282.