hnRNP I Antibody (A-4): sc-515282

hnRNP I Antibody (A-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects hnRNP I in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-hnRNP I antibody (A-4) is available in a non-conjugated form, allowing for versatile experimental use. hnRNP I, also known as polypyrimidine tract-binding protein (PTB), plays a crucial role in regulating pre-mRNA splicing and transporting mature mRNA to the cytoplasm, which is essential for proper gene expression and cellular function. hnRNP I is primarily localized in the nucleoplasm, where hnRNP I interacts with heterogeneous nuclear RNA (hnRNA) produced by RNA polymerase II, and serves as a key component of the spliceosome, a complex responsible for pre-mRNA splicing. hnRNP I′s ability to modulate alternative splicing mechanisms is particularly important in both normal cellular processes and viral infections, such as hepatitis C virus (HCV), where hnRNP I influences viral RNA translation. The human hnRNP I gene is located on chromosome 19p13.3, underscoring its significance in genetic regulation and cellular dynamics.

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hnRNP I Antibody (A-4) References:

1. Organization of the human gene encoding heterogeneous nuclear ribonucleoprotein type I (hnRNP I) and characterization of hnRNP I related pseudogene.  |  Romanelli, MG., et al. 2000. Gene. 255: 267-72. PMID: 11024286
2. Inflammation modulates the interaction of heterogeneous nuclear ribonucleoprotein (hnRNP) I/polypyrimidine tract binding protein and hnRNP L with the 3'untranslated region of the murine inducible nitric-oxide synthase mRNA.  |  Söderberg, M., et al. 2002. Mol Pharmacol. 62: 423-31. PMID: 12130696
3. hnRNP I, the polypyrimidine tract-binding protein: distinct nuclear localization and association with hnRNAs.  |  Ghetti, A., et al. 1992. Nucleic Acids Res. 20: 3671-8. PMID: 1641332
4. hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.  |  Paradis, C., et al. 2007. RNA. 13: 1287-300. PMID: 17548433
5. Linc-RoR promotes c-Myc expression through hnRNP I and AUF1.  |  Huang, J., et al. 2016. Nucleic Acids Res. 44: 3059-69. PMID: 26656491
6. hnRNP I regulates neonatal immune adaptation and prevents colitis and colorectal cancer.  |  Jin, Z., et al. 2017. PLoS Genet. 13: e1006672. PMID: 28296893
7. Long noncoding RNA UCA1 promotes glutamine-driven anaplerosis of bladder cancer by interacting with hnRNP I/L to upregulate GPT2 expression.  |  Zhao, H., et al. 2022. Transl Oncol. 17: 101340. PMID: 35021150
8. HNRNP I promotes IRAK1 degradation to reduce podocyte apoptosis and inflammatory response alleviating renal injury in diabetic nephropathy.  |  Rao, Z., et al. 2024. Immunobiology. 229: 152835. PMID: 38986278
9. Sequence determinants for hnRNP I protein nuclear localization.  |  Romanelli, MG., et al. 1997. Exp Cell Res. 235: 300-4. PMID: 9281380
10. Heterogeneous nuclear ribonucleoprotein I (hnRNP-I/PTB) selectively binds the conserved 3' terminus of hepatitis C viral RNA.  |  Chung, RT. and Kaplan, LM. 1999. Biochem Biophys Res Commun. 254: 351-62. PMID: 9918842

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hnRNP I Antibody (A-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-hnRNP I Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hnRNP I Antibody (A-4) Specifications

Alternate Product Name: hnRNP I

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 291-306

Epitope Target: hnRNP I

Epitope Species: human

Product Reactivity/Detection: hnRNP I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for hnRNP I Antibody (A-4): P: Blocking Peptide

The preferred hnRNP I antibody is hnRNP I Antibody (SH54): sc-56701.