hnRNP E1 Antibody (F-6): sc-393076

hnRNP E1 Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects hnRNP E1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-hnRNP E1 antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1) plays a crucial role in regulating mRNA metabolism, including splicing, transport, and translation. hnRNP E1 is primarily located in the nucleus, where hnRNP E1 associates with pre-mRNA and hnRNA, but can also shuttle to the cytoplasm, highlighting dual functionality. This shuttling capability allows participation in translational regulation, influencing stability and translation of specific mRNAs, which is essential for proper gene expression and cellular stress response. hnRNP E1 interaction with other proteins, such as hnRNP E2, further underscores importance in mRNA stability and translation, particularly during viral infections, where hnRNP E1 can enhance viral mRNA translation. hnRNP E1 versatility in both nucleus and cytoplasm makes hnRNP E1 vital for post-transcriptional regulation of gene expression, providing insights into various cellular processes and disease mechanisms.

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hnRNP E1 Antibody (F-6) References:

1. Suppression of 15-lipoxygenase synthesis by hnRNP E1 is dependent on repetitive nature of LOX mRNA 3'-UTR control element DICE.  |  Reimann, I., et al. 2002. J Mol Biol. 315: 965-74. PMID: 11827469
2. Novel progerin-interactive partner proteins hnRNP E1, EGF, Mel 18, and UBC9 interact with lamin A/C.  |  Zhong, N., et al. 2005. Biochem Biophys Res Commun. 338: 855-61. PMID: 16248985
3. Heterogeneous nuclear ribonucleoprotein (hnRNP) E1 binds to hnRNP A2 and inhibits translation of A2 response element mRNAs.  |  Kosturko, LD., et al. 2006. Mol Biol Cell. 17: 3521-33. PMID: 16775011
4. hnRNP E1 and E2 have distinct roles in modulating HIV-1 gene expression.  |  Woolaway, K., et al. 2007. Retrovirology. 4: 28. PMID: 17451601
5. TGF-beta-mediated phosphorylation of hnRNP E1 induces EMT via transcript-selective translational induction of Dab2 and ILEI.  |  Chaudhury, A., et al. 2010. Nat Cell Biol. 12: 286-93. PMID: 20154680
6. Heterogeneous nuclear ribonucleoproteins (hnRNPs) in cellular processes: Focus on hnRNP E1's multifunctional regulatory roles.  |  Chaudhury, A., et al. 2010. RNA. 16: 1449-62. PMID: 20584894
7. Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 protects against antisense RNA and microRNAs.  |  Ho, JJ., et al. 2013. Mol Cell Biol. 33: 2029-46. PMID: 23478261
8. Translational regulation of inhibin βA by TGFβ via the RNA-binding protein hnRNP E1 enhances the invasiveness of epithelial-to-mesenchymal transitioned cells.  |  Howley, BV., et al. 2016. Oncogene. 35: 1725-35. PMID: 26096938
9. Evidence Favoring a Positive Feedback Loop for Physiologic Auto Upregulation of hnRNP-E1 during Prolonged Folate Deficiency in Human Placental Cells.  |  Tang, YS., et al. 2017. J Nutr. 147: 482-498. PMID: 28250194
10. Discovery of tetrahydroisoquinolineindole derivatives as first dual PRMT5 inhibitors/hnRNP E1 upregulators: Design, synthesis and biological evaluation.  |  Chu, WH., et al. 2023. Eur J Med Chem. 258: 115625. PMID: 37429083

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hnRNP E1 Antibody (F-6) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-hnRNP E1 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hnRNP E1 Antibody (F-6) Specifications

Alternate Product Name: hnRNP E1

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 171-280

Epitope Target: hnRNP E1

Epitope Species: human

Product Reactivity/Detection: hnRNP E1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for hnRNP E1 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred hnRNP E1 antibody is hnRNP E1 Antibody (E-2): sc-137249.