hnRNP E1 Antibody (E-2): sc-137249

hnRNP E1 Antibody (E-2) is a mouse monoclonal IgG3 antibody that detects hnRNP E1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-hnRNP E1 antibody (E-2) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. hnRNP E1, a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, plays a crucial role in mRNA metabolism, including transcription, pre-mRNA processing, and the transport of mature mRNA to the cytoplasm for translation. hnRNP E1 is primarily localized in the nucleus, where hnRNP E1 associates with heterogeneous nuclear RNA (hnRNA) produced by RNA polymerase II, and is essential for the formation of spliceosomal complexes that facilitate mRNA splicing. hnRNP E1 shuttles between the nucleus and cytoplasm, where hnRNP E1 may act as a translational regulatory protein, influencing the stability and translation of specific mRNAs. hnRNP E1′s interaction with other proteins, such as hnRNP E2, which stabilizes polioviral mRNA, highlights hnRNP E1′s importance in viral translation and cellular stress responses. hnRNP E1′s participation in these critical processes underscores hnRNP E1′s significance in gene expression regulation and potential implications in various diseases, making hnRNP E1 (E-2) monoclonal antibody a valuable tool for researchers studying RNA biology and related pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

hnRNP E1 Antibody (E-2) References:

1. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs.  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
2. Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm.  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
3. Spatiotemporal regulation of hnRNP M and 2H9 gene expression during mouse embryonic development.  |  Mahé, D., et al. 2000. Biochim Biophys Acta. 1492: 414-24. PMID: 11004512
4. Incrimination of heterogeneous nuclear ribonucleoprotein E1 (hnRNP-E1) as a candidate sensor of physiological folate deficiency.  |  Tang, YS., et al. 2011. J Biol Chem. 286: 39100-15. PMID: 21930702
5. TGF-beta signaling in cancer: post-transcriptional regulation of EMT via hnRNP E1.  |  Howley, BV. and Howe, PH. 2019. Cytokine. 118: 19-26. PMID: 29396052
6. hnRNP E1 at the crossroads of translational regulation of epithelial-mesenchymal transition.  |  Grelet, S. and Howe, PH. 2019. J Cancer Metastasis Treat. 5: PMID: 31681852
7. The ubiquitin E3 ligase ARIH1 regulates hnRNP E1 protein stability, EMT and breast cancer progression.  |  Howley, BV., et al. 2022. Oncogene. 41: 1679-1690. PMID: 35102251
8. hnRNP E1 Regulates HPV16 Oncogene Expression and Inhibits Cervical Cancerization.  |  Song, L., et al. 2022. Front Oncol. 12: 905900. PMID: 35800060
9. A nuclear localization domain in the hnRNP A1 protein.  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
10. Identification and characterisation of a novel human RNA-binding protein.  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
11. The human hnRNP-M proteins: structure and relation with early heat shock-induced splicing arrest and chromosome mapping.  |  Gattoni, R., et al. 1996. Nucleic Acids Res. 24: 2535-42. PMID: 8692693
12. mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end.  |  Ostareck, DH., et al. 1997. Cell. 89: 597-606. PMID: 9160751

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hnRNP E1 Antibody (E-2) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-hnRNP E1 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hnRNP E1 Antibody (E-2) Specifications

Alternate Product Name: hnRNP E1

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃

Amino Acids: 227-261

Epitope Target: hnRNP E1

Epitope Species: human

Product Reactivity/Detection: hnRNP E1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for hnRNP E1 Antibody (E-2): HRP: Horseradish Peroxidase, P: Blocking Peptide

The preferred hnRNP E1 antibody is hnRNP E1 Antibody (F-6): sc-393076.