HNF-4 alpha Antibody (H-1) recommended for detection of HNF-4α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HNF-4α Antibody (H-1): sc-374229

HNF-4α Antibody (H-1) is rated 4.3 out of 5 by 12.
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Datasheets

    • Anti-HNF-4α Antibody (H-1) is a mouse monoclonal IgG1 (kappa light chain) HNF-4α antibody provided at 200 µg/ml
    • raised against amino acids 295-465 of HNF-4α of human origin
    • Anti-HNF-4 alpha Antibody (H-1) is recommended for detection of HNF-4α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-HNF-4 alpha Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-374229 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HNF-4α (H-1): sc-374229.

Can we request a trial size of the HNF4 (H-1) (sc-374229)? We have been testing several antibody combinations to try to visualize HCC in mouse tissue while co-staining. So far, many of the goat and rat antibodies have not worked at all. Thank you.

Asked by: Ashley Haddock
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2020-05-06

What is the method (steps) of applying this clone as immunohistochemical marker to the paraffin embedded sections, please?

Asked by: Dodo123
Thank you for your question. Here is a link to our recommended protocol for IHC on paraffin tissue sections: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2020-02-17

How much antibody do I get in an order of sc-374229? All I see on the webpage is the concentration of the antibody (200 ug/ml). Do you send a ml? 

Asked by: cfb6
Thank you for your question. Yes, this antibody, sc-374229, is provided as 200µg of antibody in 1ml.
Answered by: Tech Service
Date published: 2019-06-24

Can the TransCruz reagent sc-374229 X be used in applications other than ChIP and EMSA?

Asked by: AbPolly
Thank you for your question. Yes, the X form of the antibody can be used in other applications such as Western Blot, IF and IHC. The dilution factor needs to be adjusted as the X form is ten times as concentrated as the standard antibody.
Answered by: Technical Service
Date published: 2017-01-27
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Rated 4 out of 5 by from Complutense University School of Medicine We use this antibody for IF staining with HepaRG undifferentiated cells experiment which sounds good, but not HepG2 cells.
Date published: 2019-06-07
Rated 1 out of 5 by from No signal at all in mouse liver Although HNF-4a is a well-established hepatocyte marker this antibody did not stain mouse liver sections. We tested it in paraffin-embedded mouse liver sections using PT Link (DAKO) High pH, PT Link Low pH and citrate buffer for antigen retrieval. We tested it in mouse liver cryosections too. Dilutions tested were 1:100 and 1:500. None of this conditions showed any nuclear staining in hepatocytes. Can you help me with troubleshooting? Thank you, TR22
Date published: 2018-03-12
Rated 5 out of 5 by from good work well for IHC, it marks the hepatocytes in liver section.
Date published: 2017-11-14
Rated 4 out of 5 by from A good Antibody I got a free sample a month ago and are happy that it was work on western blot in mice liver tissues. We had ordered this Ab.
Date published: 2017-10-06
Rated 4 out of 5 by from 效果不错 同样的,HNF-4a,本人也是申请的小样抗体,目前只跑了WB,1:500的浓度稀释,条带还不错,感觉有点淡,会有扎带,但不是很明显
Date published: 2017-09-22
Rated 3 out of 5 by from 感觉不怎么样 之前订购的是羊的多抗,但是现在下架了,然后用的是鼠的单抗,在肝癌和肠癌里面做了个对比,1:1000的稀释比,和以前的实验结果相比,条带是干净,但是没有以前的好显,而且现在一些肠癌里面都显不出来了,这可能和单抗没有多抗的识别位点多有关吧,建议能够解决这个问题。
Date published: 2017-06-13
Rated 5 out of 5 by from Best working for human samples I bought this few weeks ago and tested them on the xenograft tumors from HepG2 (Human HCC cell line) samples. This antibody is brilliant and I got very sharp precise staining.
Date published: 2017-02-15
Rated 5 out of 5 by from Highly recommended for IHC(P). Great Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue and human colon tissue showing nuclear staining.
Date published: 2016-12-28
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