Date published: 2025-9-7
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HNF-4α Antibody (H-1) - Immunohistochemistry - Image 353791
HNF-4α (H-1): sc-374229. 福尔马林固定, 石蜡包埋的人结肠组织的免疫过氧化物酶染色, 显示腺细胞的核染色.
HNF-4α (H-1): sc-374229. 福尔马林固定, 石蜡包埋的人结肠组织的免疫过氧化物酶染色, 显示腺细胞的核染色.

HNF-4α 抗体 (H-1): sc-374229

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说明书
  • HNF-4 alpha 抗体 H-1 是小鼠单克隆 IgG1 κ，HNF-4α抗体, 在61篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HNF-4α的氨基酸295-465
  • HNF-4 alpha 抗体 (H-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HNF-4α
  • 抗HNF-4 alpha抗体（H-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-374229 X, 200 µg/0.1 ml)
  • 关于如何获取HNF-4α (H-1): sc-374229的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 HNF-4α 抗体 (H-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HNF-4α 抗体 (H-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 HNF 抗体包括 HNF-1, HNF-1 α, HNF-1 β, HNF-3 α, HNF-3 β, HNF-3 γ, HNF-4 α 和 HNF-6

HNF-4α抗体（H-1）是一种IgG1 κ小鼠单克隆HNF-4 alpha抗体（也称为HNF4A抗体或肝细胞核因子4 alpha抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的HNF-4 alpha蛋白。HNF-4α抗体（H-1）既可以是非偶联的抗HNF-4 alpha抗体形式，也可以是多种偶联形式的抗HNF-4 alpha抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。HNF-1（α和β）、HNF-3（α、β和γ）、HNF-4（α和γ）和HNF-6部分组成了一个称为肝细胞核因子家族的同源蛋白家族。各种HNF-1异构体调节肝脏以及其他组织（如肾脏、小肠和胸腺）中的基因转录。HNF-3α、HNF-3β和HNF-3γ调节成年肝脏中众多肝细胞基因的转录。HNF-3α和HNF-3β还参与原肠胚形成事件，如体轴形成。已显示HNF-4α和HNF-4γ对早期胚胎发育很重要。HNF-4α在肝脏、肾脏、胰腺、小肠、睾丸和结肠中表达；而HNF-4γ在这些组织中除肝脏外均有表达。已显示HNF-6与HNF-3β的启动子结合，这表明HNF-6在肠道内胚层上皮细胞分化中的潜在作用。有证据表明，HNF-6还可能是至少22个其他肝细胞富集基因的转录激活剂，包括细胞色素P450 2C13和α-1抗胰蛋白酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HNF-4α 抗体 (H-1) 参考文献:

  1. 人类肠道脂肪酸结合蛋白（hFABP2）基因受 HNF-4alpha 的调控。  |  Klapper, M., et al. 2007. Biochem Biophys Res Commun. 356: 147-52. PMID: 17343826
  2. 肝细胞核因子-4{α}调节肾脏中的人类有机阴离子转运体1基因  |  Ogasawara, K., et al. 2007. Am J Physiol Renal Physiol. 292: F1819-26. PMID: 17344191
  3. 白细胞介素-1β通过抑制肝细胞核因子-4α抑制促红细胞生成素增强子的缺氧诱导性。  |  Krajewski, J., et al. 2007. Cell Mol Life Sci. 64: 989-98. PMID: 17372675
  4. 肝细胞核因子-4α是内脏内胚层的发育调节因子，其表达与胃腺癌的肠表型相关。  |  Kojima, K., et al. 2006. Pathology. 38: 548-54. PMID: 17393984
  5. 随着代谢需求的增加，成体β细胞质量的扩大依赖于 HNF-4α。  |  Gupta, RK., et al. 2007. Genes Dev. 21: 756-69. PMID: 17403778
  6. 通过替代 RNA 处理，可生成更强的转录激活剂或 HNF1 同源蛋白家族的显性抑制剂。  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
  7. 小鼠转录因子 HNF-3 基因家族：基因结构, cDNA 序列和 mRNA 分布。  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
  8. 人类肝细胞核因子 4 同工型由不同的基因编码，且表达量不同。  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
  9. 转录激活因子肝细胞核因子 6 可调节肝脏基因的表达。  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657

订购信息

产品名称产品编号规格价格数量收藏夹

HNF-4α 抗体 (H-1)

sc-374229
200 µg/ml
$316.00

HNF-4α (H-1): m-IgG Fc BP-HRP 套装

sc-529692
200 µg Ab; 10 µg BP
$354.00

HNF-4α (H-1): m-IgGκ BP-HRP 套装

sc-522666
200 µg Ab, 40 µg BP
$354.00

HNF-4α (H-1): m-IgG1 BP-HRP 套装

sc-543637
200 µg Ab; 20 µg BP
$354.00

HNF-4α 抗体 (H-1) X

sc-374229 X
200 µg/0.1 ml
$316.00

HNF-4α 抗体 (H-1) AC

sc-374229 AC
500 µg/ml, 25% agarose
$416.00

HNF-4α 抗体 (H-1) HRP

sc-374229 HRP
200 µg/ml
$316.00

HNF-4α 抗体 (H-1) FITC

sc-374229 FITC
200 µg/ml
$330.00

HNF-4α 抗体 (H-1) PE

sc-374229 PE
200 µg/ml
$343.00

HNF-4α 抗体 (H-1) Alexa Fluor® 488

sc-374229 AF488
200 µg/ml
$357.00

HNF-4α 抗体 (H-1) Alexa Fluor® 546

sc-374229 AF546
200 µg/ml
$357.00

HNF-4α 抗体 (H-1) Alexa Fluor® 594

sc-374229 AF594
200 µg/ml
$357.00

HNF-4α 抗体 (H-1) Alexa Fluor® 647

sc-374229 AF647
200 µg/ml
$357.00

HNF-4α 抗体 (H-1) Alexa Fluor® 680

sc-374229 AF680
200 µg/ml
$357.00

HNF-4α 抗体 (H-1) Alexa Fluor® 790

sc-374229 AF790
200 µg/ml
$357.00

Can you supply this product (sc-374229 AF488) in a trial size? Thank you

Asked by: Babr
Thank you for your question. At this time , we do not offer a conjugated trial size. To request an unconjugated antibody sample please contact Technical Service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-07-28

The report of the source of the epitope indicates that HNF-4a(H-1) is a mouse monoclonal antibody raised against amino acids 295-465 of HNF-4a of human origin. I'd like to know the specific amino acid sequence. What isoform of HNF4a does this refer to?

Asked by: AB12345
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-08-30

Twelve HNF4a isoforms are described in litterature (Hui Ling Ko et al, Cell Reports, 2019). Which isoform(s) recongnize sc-374229 antibody?

Asked by: Maude
Thank you for your question. HNF-4α Antibody (H-1): sc-374229 was raised against amino acids 295-465 of HNF-4α of human origin. We do not have any data indicating which isoforms the antibody recognizes based on this immunogenic sequence.
Answered by: Tech Support Europe
Date published: 2020-12-28

Can we request a trial size of the HNF4 (H-1) (sc-374229)? We have been testing several antibody combinations to try to visualize HCC in mouse tissue while co-staining. So far, many of the goat and rat antibodies have not worked at all. Thank you.

Asked by: Ashley Haddock
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2020-05-06

What is the method (steps) of applying this clone as immunohistochemical marker to the paraffin embedded sections, please?

Asked by: Dodo123
Thank you for your question. Here is a link to our recommended protocol for IHC on paraffin tissue sections: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2020-02-17

How much antibody do I get in an order of sc-374229? All I see on the webpage is the concentration of the antibody (200 ug/ml). Do you send a ml? 

Asked by: cfb6
Thank you for your question. Yes, this antibody, sc-374229, is provided as 200µg of antibody in 1ml.
Answered by: Tech Service
Date published: 2019-06-24

Can the TransCruz reagent sc-374229 X be used in applications other than ChIP and EMSA?

Asked by: AbPolly
Thank you for your question. Yes, the X form of the antibody can be used in other applications such as Western Blot, IF and IHC. The dilution factor needs to be adjusted as the X form is ten times as concentrated as the standard antibody.
Answered by: Technical Service
Date published: 2017-01-27
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Rated 1 out of 5 by from Does not work Mouse liver IHCTried multiple dilutions with no staining, as well as reached out multiple times to troubleshoot antibody with customer service with zero response after multiple attempts. Do not use.
Date published: 2023-12-11
Rated 1 out of 5 by from Not working in mouse liver by IHC/IFI didn't get any stains in the nucleus in mouse liver at all with 1:50 by both IHC and IF. I tried Alexa Fluor 488 goat anti-mouse and Alexa Fluor 594 goat anti-mouse 2nd abs, but still no signals. I contacted with the technical support. They shared me some references, no replacements and no solutions.
Date published: 2021-06-30
Rated 2 out of 5 by from Not working in MIN6 cellsWe performed an IHC in MIN6 cells (mouse) with the concentrated antibody of HNF4a (diluted 10x times to match the normal concentration) and we obtained no signal in 1:50 and 1:100 concentration. We know these cells are expressing HNF4a and the antibody should be working with mouse cells according to the datasheet. We already tested a free sample of HNF1a and the results were better, with some signal in this case. Can you help is with troubleshooting? HNF4a antibody could be dead? Kind regards, Pablo.
Date published: 2021-04-27
Rated 4 out of 5 by from Complutense University School of MedicineWe use this antibody for IF staining with HepaRG undifferentiated cells experiment which sounds good, but not HepG2 cells.
Date published: 2019-06-07
Rated 1 out of 5 by from No signal at all in mouse liverAlthough HNF-4a is a well-established hepatocyte marker this antibody did not stain mouse liver sections. We tested it in paraffin-embedded mouse liver sections using PT Link (DAKO) High pH, PT Link Low pH and citrate buffer for antigen retrieval. We tested it in mouse liver cryosections too. Dilutions tested were 1:100 and 1:500. None of this conditions showed any nuclear staining in hepatocytes. Can you help me with troubleshooting? Thank you, TR22
Date published: 2018-03-12
Rated 5 out of 5 by from goodwork well for IHC, it marks the hepatocytes in liver section.
Date published: 2017-11-14
Rated 4 out of 5 by from A good AntibodyI got a free sample a month ago and are happy that it was work on western blot in mice liver tissues. We had ordered this Ab.
Date published: 2017-10-06
Rated 4 out of 5 by from 效果不错同样的，HNF-4a，本人也是申请的小样抗体，目前只跑了WB，1:500的浓度稀释，条带还不错，感觉有点淡，会有扎带，但不是很明显
Date published: 2017-09-22
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HNF-4α 抗体 (H-1) is rated 3.7 out of 5 by 15.
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