HNF-4 alpha Antibody (Q-15) recommended for detection of HNF-4α of mouse, rat and human origin by WB, IP, IF and ELISA

HNF-4α Antibody (Q-15): sc-101059

HNF-4α Antibody (Q-15) is rated 4.3 out of 5 by 3.
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Datasheets

    • HNF-4α Antibody (Q-15) is a mouse monoclonal IgG2a (kappa light chain) provided at 50 µg/0.5 ml
    • raised against recombinant HNF-4α of human origin
    • recommended for detection of HNF-4α of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See HNF-4α (H-1): sc-374229 for HNF-4α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HNF-4α (Q-15): sc-101059.
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I am using HNF-4α (Q-15): sc-101059 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from WB The results of WB was clear,clean.And the size correct.
Date published: 2017-09-07
Rated 4 out of 5 by from IF (PFA) of HeLa cells shows good nuclear IF (PFA) of HeLa cells shows good nuclear localization -SCBT QC
Date published: 2015-04-02
Rated 4 out of 5 by from Good for Western Blot Good for Western Blot, Hep G2 whole cell lysate shows positive band -SCBT QC
Date published: 2014-02-11
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