HMG-1/HMGB1 Antibody (B-5): sc-518194

HMG-1 Antibody (B-5) is a mouse monoclonal IgG1 antibody that detects HMG-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HMG-1, a member of the high mobility group protein family, plays a crucial role in the regulation of gene expression by binding to DNA and inducing structural changes that facilitate transcription. HMG-1 is predominantly located in the nucleus, where HMG-1 interacts with chromatin and influences the architecture of DNA, making HMG-1 essential for processes such as DNA repair, replication, and transcriptional activation. The ability of HMG-1 to bend and unwind DNA is vital for the recruitment of transcription factors and other regulatory proteins, thereby enhancing the transcriptional response to various stimuli. Additionally, HMG-1 undergoes post-translational modifications, including phosphorylation, which can further modulate activity and interactions with other proteins. Anti-HMG-1 antibody (B-5) is an invaluable tool for researchers studying gene regulation, chromatin dynamics, and the molecular mechanisms underlying various cellular processes.

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HMG-1/HMGB1 Antibody (B-5) References:

1. Directional binding of HMG-I(Y) on four-way junction DNA and the molecular basis for competitive binding with HMG-1 and histone H1.  |  Hill, DA., et al. 1999. Nucleic Acids Res. 27: 2135-44. PMID: 10219086
2. High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 are significant target antigens of perinuclear anti-neutrophil cytoplasmic antibodies in autoimmune hepatitis.  |  Sobajima, J., et al. 1999. Gut. 44: 867-73. PMID: 10323891
3. The characterization of the invasion phenotype of uveal melanoma tumour cells shows the presence of MUC18 and HMG-1 metastasis markers and leads to the identification of DJ-1 as a potential serum biomarker.  |  Pardo, M., et al. 2006. Int J Cancer. 119: 1014-22. PMID: 16570276
4. Physical properties of chicken erythrocyte HMG-1, HMG-2 and HMG-E.  |  Land, MD., et al. 1985. Biochim Biophys Acta. 831: 207-12. PMID: 4041467
5. Interaction between domains in chromosomal protein HMG-1.  |  Carballo, M., et al. 1984. EMBO J. 3: 1255-61. PMID: 6086312
6. mRNA of chromosomal proteins HMG-1 and HMG-2 are polyadenylated.  |  Bustin, M., et al. 1981. Biochem Biophys Res Commun. 101: 893-7. PMID: 6118142
7. Increased DNA-bending activity and higher affinity DNA binding of high mobility group protein HMG-1 prepared without acids.  |  Wagner, JP., et al. 1995. J Biol Chem. 270: 7394-8. PMID: 7706284
8. Features of the hmg 1 subfamily of genes encoding HMG-CoA reductase in potato.  |  Bhattacharyya, MK., et al. 1995. Plant Mol Biol. 28: 1-15. PMID: 7787174
9. Region of insect high mobility group (HMG) 1 protein homologous to helix 2 of the rat HMG1-b box is in close contact with DNA.  |  Wiśniewski, JR., et al. 1994. J Biol Chem. 269: 29261-4. PMID: 7961895
10. Calcium binding to HMG1 protein induces DNA looping by the HMG-box domains.  |  Stros, M., et al. 1994. FEBS Lett. 344: 201-6. PMID: 8187884

The preferred HMG-1/HMGB1 antibody is HMG-1/HMGB1 Antibody (D-3): sc-518191.