Anti-Histone H1 Antibody (H-2) recommended for detection of all histone H1 isoforms of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Histone H1 Antibody (H-2): sc-393358

Anti-Histone H1 Antibody (H-2) is rated 4.3 out of 5 by 7.
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Datasheets

    • Anti-Histone H1 Antibody (H-2) is a mouse monoclonal IgG1 λ Histone H1 antibody, cited in 20 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 22-47 near the N-terminus of Histone H1 of human origin
    • Anti-Histone H1 Antibody (H-2) is recommended for detection of all histone H1 isoforms of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Histone H1 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-393358 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Histone H1 (H-2): sc-393358.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Histone H1 Antibody (H-2) for WB applications. This reagent is now offered in a bundle with Histone H1 Antibody (H-2) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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When should the TransCruz reagent sc-393358 X be used instead of the standard form sc-393358?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 7
Date published: 2017-01-13

What secondary is best to use with Histone H1 (H-2): sc-393358?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Histone H1 (H-2):sc-393358, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 1 out of 5 by from Not good for IF Weak signal for IF in hunam HT1080 cells at 1:100 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Good for WB Strong single band - worked well in nuclear fractionation WB.
Date published: 2017-09-13
Rated 5 out of 5 by from Works great for Western Blot. Used at a concentration of 1:2000 for Western blot with mouse tissues. Worked very well, clean blot and a single band. I would recommend!
Date published: 2017-09-01
Rated 5 out of 5 by from Superb !! Great product for western blot applications; strong signal
Date published: 2017-01-20
Rated 5 out of 5 by from good in WB This Histone H1 antibody shows specific strong bands in WB.
Date published: 2017-01-12
Rated 4 out of 5 by from Potent and very specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~32-33 kDa target protein in a HeLa/Jurkat cell lysate mix, but not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using A-431, LNCaP, HeLa and HL-60 whole cell lysates and Jurkat nuclear extract. -SCBT QC
Date published: 2014-07-05
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