HIP1 Antibody (H-6): sc-271341

HIP1 Antibody (H-6) is a mouse monoclonal IgG1 antibody that detects HIP1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HIP1, or huntingtin-interacting protein 1, plays a critical role in maintaining cellular integrity, particularly in the brain, where HIP1 is involved in vesicle trafficking and membrane-cytoskeletal interactions. HIP1 is predominantly located in the cytoplasm and is essential for proper functioning of synaptic vesicles, which are vital for neurotransmitter release and communication between neurons. HIP1′s structural integrity is crucial, as HIP1 contains a C-terminal actin-binding domain, a central coiled-coil region, and an N-terminal ENTH domain, all of which contribute to HIP1′s function in vesicle size maintenance and trafficking. Disruption of HIP1′s function can lead to compromised cellular processes, particularly in neurodegenerative diseases such as Huntington′s disease, where HIP1′s interaction with huntingtin is inversely correlated with the length of polyglutamine repeats. Anti-HIP1 antibody (H-6) is an invaluable tool for researchers studying vesicle trafficking dynamics and molecular mechanisms underlying neurodegeneration, providing insights into HIP1′s critical roles in neuronal health and disease.

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HIP1 Antibody (H-6) References:

1. An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles.  |  Engqvist-Goldstein, AE., et al. 1999. J Cell Biol. 147: 1503-18. PMID: 10613908
2. Recruitment and activation of caspase-8 by the Huntingtin-interacting protein Hip-1 and a novel partner Hippi.  |  Gervais, FG., et al. 2002. Nat Cell Biol. 4: 95-105. PMID: 11788820
3. Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo.  |  Chen, CY. and Brodsky, FM. 2005. J Biol Chem. 280: 6109-17. PMID: 15533940
4. Degenerative phenotypes caused by the combined deficiency of murine HIP1 and HIP1r are rescued by human HIP1.  |  Bradley, SV., et al. 2007. Hum Mol Genet. 16: 1279-92. PMID: 17452370
5. Accommodation of structural rearrangements in the huntingtin-interacting protein 1 coiled-coil domain.  |  Wilbur, JD., et al. 2010. Acta Crystallogr D Biol Crystallogr. 66: 314-8. PMID: 20179344
6. Huntingtin-interacting protein 1-related is required for accurate congression and segregation of chromosomes.  |  Park, SJ. 2010. BMB Rep. 43: 795-800. PMID: 21189155
7. Regulation of RE1 protein silencing transcription factor (REST) expression by HIP1 protein interactor (HIPPI).  |  Datta, M. and Bhattacharyya, NP. 2011. J Biol Chem. 286: 33759-69. PMID: 21832040
8. Knockdown of HIP1 expression promotes ligand‑induced endocytosis of EGFR in HeLa cells.  |  Li, D., et al. 2017. Oncol Rep. 38: 3387-3391. PMID: 29039605
9. Targeted inhibition of NRF2 reduces the invasive and metastatic ability of HIP1 depleted lung cancer cells.  |  Prasad, P., et al. 2024. Biochem Biophys Res Commun. 733: 150676. PMID: 39303527
10. Cloning, expression analysis, and chromosomal localization of HIP1R, an isolog of huntingtin interacting protein (HIP1).  |  Seki, N., et al. 1998. J Hum Genet. 43: 268-71. PMID: 9852681

The preferred HIP1 antibody is HIP1 Antibody (4B10): sc-47754.