HIP1 Antibody (4B10): sc-47754

HIP1 Antibody (4B10) is a mouse monoclonal IgG1 kappa light chain antibody that detects HIP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. HIP1 (4B10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIP1, known as huntingtin-interacting protein 1, plays a critical role in cellular processes, particularly in Huntington′s disease, where interaction with huntingtin is essential for maintaining membrane-cytoskeletal integrity in the brain. HIP1 is a membrane-associated protein that binds specifically to the N-terminus of huntingtin, with expression observed in various brain regions at low levels. The structural integrity of HIP1 is vital for function, containing an endocytic multidomain structure with a C-terminal actin-binding domain, a central coiled-coil region, and an N-terminal ENTH domain, which are all important for vesicle trafficking. Notably, interaction between huntingtin and HIP1 is inversely correlated with the length of the polyglutamine tract in huntingtin, suggesting that alterations in this interaction may contribute to the pathophysiology of Huntington′s disease. Additionally, HIP12, a non-proapoptotic member of the HIP gene family, shares a similar subcellular distribution pattern with HIP1 but does not directly interact with huntingtin, although HIP12 can interact with HIP1, highlighting the complex interplay of these proteins in neuronal function.

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HIP1 Antibody (4B10) References:

1. HIP12 is a non-proapoptotic member of a gene family including HIP1, an interacting protein with huntingtin.  |  Chopra, VS., et al. 2000. Mamm Genome. 11: 1006-15. PMID: 11063258
2. The huntingtin interacting protein HIP1 is a clathrin and alpha-adaptin-binding protein involved in receptor-mediated endocytosis.  |  Waelter, S., et al. 2001. Hum Mol Genet. 10: 1807-17. PMID: 11532990
3. Huntingtin interacting protein 1 as a histopathologic adjunct in the diagnosis of Merkel cell carcinoma.  |  Marghalani, S., et al. 2015. Int J Dermatol. 54: 640-7. PMID: 25070010
4. Prognostic significance of huntingtin interacting protein 1 expression on patients with acute myeloid leukemia.  |  Wang, J., et al. 2017. Sci Rep. 7: 45960. PMID: 28452374
5. Huntingtin-interacting protein 1 (HIP1) regulates arthritis severity and synovial fibroblast invasiveness by altering PDGFR and Rac1 signalling.  |  Laragione, T., et al. 2018. Ann Rheum Dis. 77: 1627-1635. PMID: 30049830
6. Huntingtin-Interacting Protein 1 Promotes Vpr-Induced G2 Arrest and HIV-1 Infection in Macrophages.  |  Murakami, T., et al. 2021. Viruses. 13: PMID: 34835114
7. Y-box protein-1 modulates circSPECC1 to promote glioma tumorigenesis via miR-615-5p/HIP1/AKT axis.  |  Lan, P., et al. 2023. Acta Biochim Biophys Sin (Shanghai). 55: 1902-1912. PMID: 37994157
8. KIF1C and new Huntingtin-interacting protein 1 binding proteins regulate rheumatoid arthritis fibroblast-like synoviocytes' phenotypes.  |  Laragione, T., et al. 2024. Front Immunol. 15: 1323410. PMID: 38726004
9. HIP1, a human homologue of S. cerevisiae Sla2p, interacts with membrane-associated huntingtin in the brain.  |  Kalchman, MA., et al. 1997. Nat Genet. 16: 44-53. PMID: 9140394
10. HIP-I: a huntingtin interacting protein isolated by the yeast two-hybrid system.  |  Wanker, EE., et al. 1997. Hum Mol Genet. 6: 487-95. PMID: 9147654
11. Localization of the human HIP1 gene close to the elastin (ELN) locus on 7q11.23.  |  Wedemeyer, N., et al. 1997. Genomics. 46: 313-5. PMID: 9417924
12. IRS-PCR-based genetic mapping of the huntingtin interacting protein gene (HIP1) on mouse chromosome 5.  |  Himmelbauer, H., et al. 1998. Mamm Genome. 9: 26-31. PMID: 9434941

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HIP1 Antibody (4B10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-HIP1 Antibody (4B10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HIP1 Antibody (4B10) Specifications

Alternate Product Name: HIP1

Product Clone Name: 4B10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: HIP1

Epitope Species: human

Product Reactivity/Detection: HIP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for HIP1 Antibody (4B10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin