HIF-3α Antibody (D-7): sc-390769

HIF-3α Antibody (D-7) is a mouse monoclonal IgG2b antibody that detects HIF-3α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HIF-3α, a member of the hypoxia-inducible factor family, plays a crucial role in cellular responses to low oxygen levels, acting as a transcription factor that regulates the expression of genes involved in various adaptive processes, including metabolism and angiogenesis. Unlike HIF-1α and HIF-2α, HIF-3α has distinct regulatory functions, including the modulation of gene expression in response to hypoxia, which is vital for maintaining cellular homeostasis under stress conditions. HIF-3α is primarily located in the nucleus, where HIF-3α forms heterodimers with the aryl hydrocarbon receptor nuclear translocator (Arnt) to bind to hypoxia-responsive elements in target gene promoters. This nuclear localization enables HIF-3α to effectively regulate the transcription of genes that facilitate adaptation to hypoxic environments, such as those involved in erythropoiesis and glucose metabolism. HIF-3α interactions with other proteins in the hypoxia signaling pathway further underscore anti-HIF-3α antibody (D-7)′s importance as a valuable tool for researchers studying hypoxia-related processes and diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HIF-3α Antibody (D-7) References:

1. The action of HIF-3α variants on HIF-2α-HIF-1β heterodimer formation is directly probed in live cells.  |  Kim, SH., et al. 2015. Exp Cell Res. 336: 329-37. PMID: 26160453
2. Parkin modulates expression of HIF-1α and HIF-3α during hypoxia in gliobastoma-derived cell lines in vitro.  |  Maugeri, G., et al. 2016. Cell Tissue Res. 364: 465-474. PMID: 26742768
3. An oxygen-insensitive Hif-3α isoform inhibits Wnt signaling by destabilizing the nuclear β-catenin complex.  |  Zhang, P., et al. 2016. Elife. 5: PMID: 26765566
4. HIF-3α Promotes Metastatic Phenotypes in Pancreatic Cancer by Transcriptional Regulation of the RhoC-ROCK1 Signaling Pathway.  |  Zhou, X., et al. 2018. Mol Cancer Res. 16: 124-134. PMID: 28928287
5. Nuclear localization of Hif-3α requires two redundant NLS motifs in its unique C-terminal region.  |  Yao, Q., et al. 2018. FEBS Lett. 592: 2769-2775. PMID: 30047986
6. HIF‑3α affects preeclampsia development by regulating EVT growth via activation of the Flt‑1/JAK/STAT signaling pathway in hypoxia.  |  Qu, H., et al. 2021. Mol Med Rep. 23: PMID: 33215219
7. A Functional Polymorphism in HIF-3α Is Related to an Increased Risk of Ischemic Stroke.  |  Gu, XX., et al. 2021. J Mol Neurosci. 71: 1061-1069. PMID: 33226577
8. miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone.  |  Ouyang, L., et al. 2022. iScience. 25: 103604. PMID: 35005549
9. Identification of oleoylethanolamide as an endogenous ligand for HIF-3α.  |  Diao, X., et al. 2022. Nat Commun. 13: 2529. PMID: 35534502
10. Hypoxia inducible factor (HIF) 3α prevents COPD by inhibiting alveolar epithelial cell ferroptosis via the HIF-3α-GPx4 axis.  |  Jiang, J., et al. 2024. Theranostics. 14: 5512-5527. PMID: 39310101

The preferred HIF-3α antibody is HIF-3α Antibody (E-8): sc-390933.