HIF-3α Antibody (E-8): sc-390933

HIF-3α Antibody (E-8) is a mouse monoclonal IgM antibody that detects HIF-3α protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HIF-3α antibody (E-8) is available as a non-conjugated format. HIF-3α protein plays a crucial role in cellular responses to hypoxia, which is a condition characterized by low oxygen levels. HIF-3α protein is primarily located in the nucleus, where HIF-3α functions as a transcription factor that regulates the expression of genes involved in the adaptive response to hypoxic stress. This nuclear localization allows HIF-3α protein to directly influence the transcription of target genes that promote processes such as angiogenesis, erythropoiesis, and metabolic adaptation, thereby helping cells survive in low-oxygen environments. HIF-3α protein can form heterodimers with other hypoxia-inducible factors, such as HIF-1α and EPAS-1, enhancing regulatory capacity and ensuring a coordinated response to hypoxia. HIF-3α protein′s ability to modulate gene expression in response to oxygen levels underscores importance in various physiological and pathological processes, including cancer progression and cardiovascular diseases.

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HIF-3α Antibody (E-8) References:

1. Hypoxia-induced angiogenesis during carcinogenesis.  |  Choi, KS., et al. 2003. J Biochem Mol Biol. 36: 120-7. PMID: 12542982
2. Hypoxia-inducible factor (HIF-1)alpha: its protein stability and biological functions.  |  Lee, JW., et al. 2004. Exp Mol Med. 36: 1-12. PMID: 15031665
3. Roles of the human hypoxia-inducible factor (HIF)-3α variants in the hypoxia response.  |  Heikkilä, M., et al. 2011. Cell Mol Life Sci. 68: 3885-901. PMID: 21479871
4. Differential regulation of HIF-3α in LPS-induced BV-2 microglial cells: Comparison and characterization with HIF-1α.  |  Kumar, H., et al. 2015. Brain Res. 1610: 33-41. PMID: 25847716
5. Deterioration of alveolar development in mice with both HIF-3α knockout and HIF-2α knockdown.  |  Amin, FZ., et al. 2018. BMC Res Notes. 11: 449. PMID: 29986746
6. HIF-3α Facilitates the Proliferation and Migration in Pancreatic Cancer by Inhibiting Autophagy Through Downregulating TP53INP2.  |  Zhou, X., et al. 2024. Cell Biochem Biophys.. PMID: 39614944
7. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension.  |  Wang, GL., et al. 1995. Proc Natl Acad Sci U S A. 92: 5510-4. PMID: 7539918
8. Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells.  |  Tian, H., et al. 1997. Genes Dev. 11: 72-82. PMID: 9000051
9. Molecular characterization of the murine Hif-1 alpha locus.  |  Luo, G., et al. 1997. Gene Expr. 6: 287-99. PMID: 9368100
10. Selection and analysis of a mutant cell line defective in the hypoxia-inducible factor-1 alpha-subunit (HIF-1alpha). Characterization of hif-1alpha-dependent and -independent hypoxia-inducible gene expression.  |  Wood, SM., et al. 1998. J Biol Chem. 273: 8360-8. PMID: 9525945
11. Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis.  |  Carmeliet, P., et al. 1998. Nature. 394: 485-90. PMID: 9697772
12. Molecular characterization and chromosomal localization of a third alpha-class hypoxia inducible factor subunit, HIF3alpha.  |  Gu, YZ., et al. 1998. Gene Expr. 7: 205-13. PMID: 9840812

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HIF-3α Antibody (E-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-HIF-3α Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HIF-3α Antibody (E-8) Specifications

Alternate Product Name: HIF-3α

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 612-639

Epitope Target: HIF-3α

Epitope Species: mouse

Product Reactivity/Detection: HIF-3α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for HIF-3α Antibody (E-8): P: Blocking Peptide, X: TransCruz Reagent