HIF1a Antibody (28b): sc-13515

HIF-1α Antibody (28b) is a mouse monoclonal IgG1 kappa light chain antibody designed to target the hypoxia-inducible factor 1-alpha (HIF-1α) protein, with epitope mapping within amino acids 329-530 of human HIF-1α. HIF-1α monoclonal antibody (28b) is indispensable for researchers investigating cellular mechanisms of oxygen deprivation, as HIF-1α, also known as HIF1, MOP1, PASD8, and HIF-1alpha, is a pivotal transcription factor that regulates expression of genes involved in glycolysis, erythropoiesis, and angiogenesis, thereby playing a crucial role in restoring oxygen homeostasis and maintaining cell growth and viability under hypoxic conditions. HIF-1α monoclonal antibody (28b) exhibits broad species reactivity, effectively detecting HIF-1α in mouse, rat, and human samples, as well as in additional species including bovine and porcine, across various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). Available in both non-conjugated form and multiple conjugated variants, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and several Alexa Fluor® conjugates, HIF-1α monoclonal antibody (28b) provides versatile options to accommodate diverse experimental requirements. By enabling precise detection and study of HIF-1α, HIF-1α monoclonal antibody (28b) supports research into regulation of hypoxia-responsive genes and intricate pathways that cells utilize to adapt to low oxygen environments.

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HIF1a Antibody (28b) References:

1. PKB/Akt activation inhibits p53-mediated HIF1A degradation that is independent of MDM2.  |  Choy, MK., et al. 2010. J Cell Physiol. 222: 635-9. PMID: 19950214
2. STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy.  |  Ferreira, JV., et al. 2013. Autophagy. 9: 1349-66. PMID: 23880665
3. Distinct roles of HIF1A in endothelial adaptations to physiological and ambient oxygen.  |  Jiang, YZ., et al. 2014. Mol Cell Endocrinol. 391: 60-7. PMID: 24796659
4. Golgi Associated HIF1a Serves as a Reserve in Melanoma Cells.  |  Lakhter, AJ., et al. 2016. J Cell Biochem. 117: 853-9. PMID: 26375488
5. LIMS1 Promotes Pancreatic Cancer Cell Survival under Oxygen-Glucose Deprivation Conditions by Enhancing HIF1A Protein Translation.  |  Huang, C., et al. 2019. Clin Cancer Res. 25: 4091-4103. PMID: 30679163
6. Protein co-expression network-based profiles revealed from laser-microdissected cancerous cells of lung squamous-cell carcinomas.  |  Nishimura, T., et al. 2021. Sci Rep. 11: 20209. PMID: 34642392
7. CARM1 drives triple-negative breast cancer progression by coordinating with HIF1A.  |  Feng, D., et al. 2024. Protein Cell. 15: 744-765. PMID: 38476024
8. HIF1A protein expression is correlated with clinical features in gastric cancer: an updated systematic review and meta-analysis.  |  Nam, S. and Lee, Y. 2024. Sci Rep. 14: 13736. PMID: 38877062
9. Quercetin relieves compression-induced cell death and lumbar disc degeneration by stabilizing HIF1A protein.  |  Ren, J., et al. 2024. Heliyon. 10: e37349. PMID: 39296087
10. RNA binding protein ELAVL1-mediated USP33 stabilizes HIF1A to promote pathological proliferation, migration and angiogenesis of RECs.  |  Xie, J., et al. 2024. Int Ophthalmol. 44: 393. PMID: 39320536

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HIF1a Antibody (28b) has 290 citations in a variety of scientific publications.

Primary antibodies like Anti-HIF1a Antibody (28b) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HIF1a Antibody (28b) Specifications

Alternate Product Name: HIF-1α

Product Clone Name: 28b

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 329-530

Epitope Target: HIF-1α

Epitope Species: human

Product Reactivity/Detection: HIF-1α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 290 citations in various scientific publications

Available Conjugates/Forms for HIF1a Antibody (28b): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred HIF1a antibody is HIF1a Antibody (H1alpha 67): sc-53546.