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HIF1a Antibody (28b) - Immunohistochemistry - Image 348045
HIF-1α 抗体 (28b): sc-13515. 福尔马林固定, 石蜡包埋的人类睾丸组织的免疫过氧化物酶染色, 显示曲细精管细胞的核染色和Leydig细胞的胞浆染色.
HIF-1α 抗体 (28b): sc-13515. 福尔马林固定, 石蜡包埋的人类睾丸组织的免疫过氧化物酶染色, 显示曲细精管细胞的核染色和Leydig细胞的胞浆染色.

HIF1a 抗体 (28b): sc-13515

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说明书
  • HIF1a 抗体 28b 是小鼠单克隆 IgG1 κ，HIF1a抗体, 在290篇文献中引用，规格为200 µg/ml
  • 表位定位到HIF-1α的329-530氨基酸，该氨基酸来自human
  • HIF1a 抗体 (28b) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 HIF-1α; 也和以下物种反应，包括： 和 bovine and porcine
  • 抗HIF1a抗体（28b）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13515 X, 200 µg/0.1 ml)
  • 关于如何获取HIF1a (28b): sc-13515的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 HIF1a 抗体 (28b) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HIF1a 抗体 (28b) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HIF 抗体 包括 HIF-3 α

HIF-1α抗体（28b）是一种小鼠单克隆IgG1 kappa轻链抗体，专门针对缺氧诱导因子1-α（HIF-1α）蛋白，其表位位于人类HIF-1α的氨基酸329-530之间。HIF-1α 单克隆抗体（28b）对于研究缺氧细胞机制的研究人员来说是必不可少的，因为HIF-1α（也称为HIF1、MOP1、PASD8和HIF-1alpha）是一种关键的转录因子，可调节参与糖酵解、红细胞生成和血管生成的基因的表达，从而在恢复低氧条件下的氧稳态以及维持细胞生长和活力方面发挥关键作用。HIF-1α单克隆抗体（28b）具有广泛的物种反应性，可有效检测小鼠、大鼠和人类样本中的HIF-1α，以及牛和猪等其他物种的HIF-1α，适用于多种应用，如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC）。HIF-1α单克隆抗体（28b）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和几种Alexa Fluor®结合物，可满足各种实验需求。HIF-1α单克隆抗体（28b）能够精确检测和研究HIF-1α，有助于研究缺氧反应基因的调控以及细胞适应低氧环境的复杂途径。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HIF1a 抗体 (28b) 参考文献:

  1. PKB/Akt激活抑制p53介导的HIF1A降解，该降解与MDM2无关。  |  Choy, MK., et al. 2010. J Cell Physiol. 222: 635-9. PMID: 19950214
  2. STUB1/CHIP 是伴侣介导的自噬作用降解 HIF1A 所必需的。  |  Ferreira, JV., et al. 2013. Autophagy. 9: 1349-66. PMID: 23880665
  3. HIF1A在血管内皮适应生理和周围氧环境中的不同作用。  |  Jiang, YZ., et al. 2014. Mol Cell Endocrinol. 391: 60-7. PMID: 24796659
  4. 高尔基相关HIF1a在黑色素瘤细胞中起到储备作用。  |  Lakhter, AJ., et al. 2016. J Cell Biochem. 117: 853-9. PMID: 26375488
  5. LIMS1 通过增强 HIF1A 蛋白翻译促进胰腺癌细胞在氧-葡萄糖剥夺条件下的存活  |  Huang, C., et al. 2019. Clin Cancer Res. 25: 4091-4103. PMID: 30679163
  6. 基于蛋白质共表达网络的肺鳞状细胞癌激光微切割癌细胞剖面图。  |  Nishimura, T., et al. 2021. Sci Rep. 11: 20209. PMID: 34642392
  7. CARM1通过与HIF1A协调来驱动三阴性乳腺癌的进展。  |  Feng, D., et al. 2024. Protein Cell. 15: 744-765. PMID: 38476024
  8. HIF1A蛋白的表达与胃癌的临床特征相关：最新系统回顾和荟萃分析。  |  Nam, S. and Lee, Y. 2024. Sci Rep. 14: 13736. PMID: 38877062
  9. 槲皮素通过稳定HIF1A蛋白，缓解压迫引起的细胞死亡和腰椎间盘退化。  |  Ren, J., et al. 2024. Heliyon. 10: e37349. PMID: 39296087
  10. RNA结合蛋白ELAVL1介导的USP33稳定HIF1A，促进REC的病理增生、迁移和血管生成。  |  Xie, J., et al. 2024. Int Ophthalmol. 44: 393. PMID: 39320536

订购信息

产品名称产品编号规格价格数量收藏夹

HIF1a 抗体 (28b)

sc-13515
200 µg/ml
$316.00

HIF1a (28b): m-IgG Fc BP-HRP 套装

sc-528283
200 µg Ab; 10 µg BP
$354.00

HIF1a (28b): m-IgGκ BP-HRP 套装

sc-520622
200 µg Ab, 40 µg BP
$354.00

HIF1a 抗体 (28b) X

sc-13515 X
200 µg/0.1 ml
$316.00

HIF1a 抗体 (28b) AC

sc-13515 AC
500 µg/ml, 25% agarose
$416.00

HIF1a 抗体 (28b) HRP

sc-13515 HRP
200 µg/ml
$316.00

HIF1a 抗体 (28b) FITC

sc-13515 FITC
200 µg/ml
$330.00

HIF1a 抗体 (28b) PE

sc-13515 PE
200 µg/ml
$343.00

HIF1a 抗体 (28b) Alexa Fluor® 488

sc-13515 AF488
200 µg/ml
$357.00

HIF1a 抗体 (28b) Alexa Fluor® 546

sc-13515 AF546
200 µg/ml
$357.00

HIF1a 抗体 (28b) Alexa Fluor® 594

sc-13515 AF594
200 µg/ml
$357.00

HIF1a 抗体 (28b) Alexa Fluor® 647

sc-13515 AF647
200 µg/ml
$357.00

HIF1a 抗体 (28b) Alexa Fluor® 680

sc-13515 AF680
200 µg/ml
$357.00

HIF1a 抗体 (28b) Alexa Fluor® 790

sc-13515 AF790
200 µg/ml
$357.00

请问该抗体能应用于猪细胞吗？

Asked by: jchh
感谢您的提问。HIF1a 抗体 (28b): sc-13515 可以应用于的猪细胞样本。 Thank you for your question. HIF1a Antibody (28b): sc-13515 is recommended for porcine cells.
Answered by: Technical Service SH
Date published: 2024-03-25

What is the storage buffer for this antibody? Is it carrier-free? Can it be used for protein conjugation?

Asked by: Lily
Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Service
Date published: 2024-03-28

Estoy utilizando el anticuerpo sc-13515 en tejido de ratón (cerebro y placenta, con 40 y 60ug de proteína) con una concentración de Ac 1:200 pero no consigo que me salga la banda. ¿Tenéis alguna recomendación para mejorarlo?

Asked by: AGM7
Gracias por su pregunta. Recomendamos incubar con el primario durante la noche a +4 ° C para obtener resultados óptimos. Compruebe también si su anticuerpo secundario es compatible con los anticuerpos primarios IgG1 de ratón. También puede encontrar nuestro protocolo recomendado en este enlace: https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2021-07-23

what is the starting dilution for HIF-1 # (28b) and HIF-1 (H 1# 67) using IHC formalin fixed human tissues

Asked by: Anonymous
Thank you for your question. You can find this information in the antibodies' datasheets, which are available from their respective product pages. The recommended starting dilution for IHC applications is 1:50, with a dilution range between 1:50 and 1:500 depending on your samples and pre-treatment procedures.
Answered by: Tech Support Europe
Date published: 2020-08-04

你好，可以做小鼠脑片的免疫荧光吗？效果如何？

Asked by: xiaomimi
Thank you for your question. sc-13515 is recommended for detection of HIF-1α by IF on mouse sample.Please contact our Technical Service Department for more details. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website. 感谢您的提问，sc-13515 推荐免疫荧光的实验检测小鼠样本。 更多问题请请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2020-03-25

パラフィン切片を免疫組織化学染色する際は、どのような抗原賦活化を行えばよいですか？

Asked by: seigo
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to answer any questions. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2019-06-05

请问这个做肿瘤切片染色，需要二抗吗？

Asked by: Curry
感谢您的提问。免疫组化实验中，如果使用HIF-1α (28b): sc-13515，需要使用相应二抗检测。或者可购买HRP偶联的HIF-1α (28b): sc-13515，货号为sc-13515 HRP，则不需要二抗。 Thank you for your inquiry. If you perform IHC with HIF-1α (28b): sc-13515, a matched secondary antibody is necessary. Or you can choose HRP conjugated primary antibody:sc-13515 HRP.
Answered by: Technical Support 9
Date published: 2019-05-22

Does it work in bEnd3 endothelial cells. Actually i applied one another HIF1a antibodies(goat) , it worked in 1:200 . So if anyone deal with bEnd3 cells, please contact me . Thank you

Asked by: endothelial
Thank you for your question. HIF-1α Antibody (28b): sc-13515 is recommended for detection of HIF-1α of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including and bovine and porcine. You can find several publications using this antibody with murine cells on its product page.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29
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Rated 4 out of 5 by from Hif-1 AbGood antibody for detecting Hif-1a in mouse tissue (IHC, 1:100).
Date published: 2024-02-12
Rated 4 out of 5 by from Good antibody for HIF1a detectionWe tested a sample of the antibody in HeLa cells and it works well!
Date published: 2023-11-09
Rated 5 out of 5 by from HIF1a Antibody works well in human and mouseWe have great experience using this antibody in human and murine tissues with in IF and with DAB staining.
Date published: 2023-05-16
Rated 5 out of 5 by from I recomend this antibodyI used this antibody for western blot application in mouse brain samples, good sensitivity!
Date published: 2023-02-02
Rated 1 out of 5 by from Not workingI tried this antibody on cell lysates of SHSY-5Y, 1:500, ECL: Westar ETA ULTRA 2.0. No signal obtained after 15 minutes of exposition.
Date published: 2022-08-12
Rated 5 out of 5 by from 一如既往的好Santa产品值得信懒,经过很多年沉淀下来的好品牌，为科研工作带来很多的便利条件，国内现货是其他国际品牌无法媲美的
Date published: 2022-07-14
Rated 5 out of 5 by from HIF1a antibody works wellThe HIF1a antibody works well in mouse tissues. The methods are described in the supplementary section attached.
Date published: 2022-07-01
Rated 3 out of 5 by from Funzionamento buonoHo usato l'anticorpo su una linea cellulare di fegato HuH7, il segnale risulta specifico ma poco sensibile
Date published: 2022-03-24
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HIF1a抗体 (28b) is rated 4.2 out of 5 by 90.
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