HEXA Antibody (D-2): sc-376777

HEXA Antibody (D-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects HEXA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). HEXA (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HEXA protein, also known as beta-Hexosaminidase A, plays a crucial role in lysosomal function by degrading GM2 ganglioside and other molecules containing terminal N-acetyl hexosamines, particularly in brain and other tissues. This enzymatic activity maintains cellular homeostasis, as GM2 ganglioside accumulation due to HEXA deficiency can lead to severe neurological disorders, such as Tay-Sachs disease, a fatal autosomal recessive lysosomal storage disorder characterized by progressive destruction of the central nervous system. HEXA protein forms a trimer composed of one α chain and two β chains (β-A and β-B), and proper functioning prevents toxic buildup of GM2 gangliosides, which can devastate neuronal health and function.

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HEXA Antibody (D-2) References:

1. Molecular basis of hexosaminidase A deficiency and pseudodeficiency in the Berks County Pennsylvania Dutch.  |  Mules, EH., et al. 1992. Hum Mutat. 1: 298-302. PMID: 1301937
2. Pathogenic significance of alpha-N-acetylgalactosaminidase activity found in the hemagglutinin of influenza virus.  |  Yamamoto, N. and Urade, M. 2005. Microbes Infect. 7: 674-81. PMID: 15848273
3. N-acetyl-beta-D-hexosaminidase from Trichomonas vaginalis: substrate specificity and activity of inhibitors.  |  Sanon, A., et al. 2005. Biomed Pharmacother. 59: 245-8. PMID: 15890491
4. Beta-hexosaminidase isoenzyme profiles in serum, plasma, platelets and mononuclear, polymorphonuclear and unfractionated total leukocytes.  |  Casal, JA., et al. 2005. Clin Biochem. 38: 938-42. PMID: 16024010
5. Sequence of DNA flanking the exons of the HEXA gene, and identification of mutations in Tay-Sachs disease.  |  Triggs-Raine, BL., et al. 1991. Am J Hum Genet. 49: 1041-54. PMID: 1833974
6. Mice doubly-deficient in lysosomal hexosaminidase A and neuraminidase 4 show epileptic crises and rapid neuronal loss.  |  Seyrantepe, V., et al. 2010. PLoS Genet. 6: e1001118. PMID: 20862357
7. Hexosaminidase-A and hexosaminidase-B: studies in Tay-Sachs' and Sandhoff's disease.  |  Srivastava, SK. and Beutler, E. 1973. Nature. 241: 463. PMID: 4122341
8. Identification of a possible subunit of hexosaminidase A and B.  |  Robinson, D., et al. 1973. Nature. 243: 415-6. PMID: 4743635
9. Characterization of heteropolymeric hexosaminidase A in human X mouse hybrid cells.  |  Chern, J., et al. 1976. Proc Natl Acad Sci U S A. 73: 3637-40. PMID: 62363
10. Deficiency of the hexosaminidase A activator protein in a case of GM2 gangliosidosis; variant AB.  |  Hechtman, P., et al. 1982. Pediatr Res. 16: 217-22. PMID: 6801612
11. Characterization of unusual hexosaminidase A (HEX A) deficient human mutants.  |  O'Brien, JS., et al. 1978. Am J Hum Genet. 30: 602-8. PMID: 747188
12. Targeted disruption of the Hexa gene results in mice with biochemical and pathologic features of Tay-Sachs disease.  |  Yamanaka, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 9975-9. PMID: 7937929

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HEXA Antibody (D-2) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-HEXA Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HEXA Antibody (D-2) Specifications

Alternate Product Name: HEXA

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 377-416

Epitope Target: HEXA

Epitope Species: human

Product Reactivity/Detection: HEXA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for HEXA Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HEXA antibody is HEXA Antibody (E-2): sc-376735.