Hephaestin Antibody (C-8): sc-393701
- Hephaestin Antibody (C-8) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 331-420 mapping within an N-terminal extracellular domain of Hephaestin of mouse origin
- Hephaestin Antibody (C-8) is recommended for detection of Hephaestin of mouse origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Hephaestin Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Hephaestin Antibody (C-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Hephaestin Antibody (C-8) (see ordering information below).
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SEE ALSO...
Hephaestin Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Hephaestin of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Hephaestin (C-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hephaestin is a single-pass type I membrane protein that belongs to the multicopper oxidase family and plays a vital role in iron metabolism. Specifically, Hephaestin functions as a copper-dependent ferroxidase, facilitating the export of iron from intestinal enterocytes into the bloodstream. This process is essential for maintaining systemic iron homeostasis, as iron remains available for various physiological functions, including hemoglobin synthesis and cellular respiration. Hephaestin′s ability to bind six copper ions per monomer is crucial for enzymatic activity, and expression is regulated by the homeobox transcription factor CDX2, which is upregulated in response to increased iron levels. Hephaestin is primarily localized in the intestine but is also found in the colon, breast tissue, bone trabecular cells, and fibroblasts, highlighting importance in both iron and copper metabolism across different tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hephaestin Antibody (C-8) | sc-393701 | 200 µg/ml | $322.00 | |||
Hephaestin Antibody (C-8): m-IgG Fc BP-HRP Bundle | sc-530546 | 200 µg Ab; 10 µg BP | $361.00 | |||
Hephaestin Antibody (C-8): m-IgGκ BP-HRP Bundle | sc-524052 | 200 µg Ab, 40 µg BP | $361.00 | |||
Hephaestin Antibody (C-8): m-IgG1 BP-HRP Bundle | sc-544120 | 200 µg Ab; 20 µg BP | $361.00 | |||
Hephaestin Antibody (C-8) AC | sc-393701 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Hephaestin Antibody (C-8) HRP | sc-393701 HRP | 200 µg/ml | $322.00 | |||
Hephaestin Antibody (C-8) FITC | sc-393701 FITC | 200 µg/ml | $336.00 | |||
Hephaestin Antibody (C-8) PE | sc-393701 PE | 200 µg/ml | $349.00 | |||
Hephaestin Antibody (C-8) Alexa Fluor® 488 | sc-393701 AF488 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-8) Alexa Fluor® 546 | sc-393701 AF546 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-8) Alexa Fluor® 594 | sc-393701 AF594 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-8) Alexa Fluor® 647 | sc-393701 AF647 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-8) Alexa Fluor® 680 | sc-393701 AF680 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-8) Alexa Fluor® 790 | sc-393701 AF790 | 200 µg/ml | $364.00 |