Hephaestin Antibody (C-7): sc-365365
- Hephaestin Antibody (C-7) is a mouse monoclonal IgG2b κ Hephaestin antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 331-420 mapping within an N-terminal extracellular domain of Hephaestin of human origin
- Hephaestin Antibody (C-7) is recommended for detection of Hephaestin of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Hephaestin Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Hephaestin Antibody (C-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Hephaestin Antibody (C-7) (see ordering information below).
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Hephaestin Antibody (C-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects Hephaestin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Hephaestin (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hephaestin is a single-pass type I membrane protein belonging to the multicopper oxidase family, and plays a vital role as a copper-dependent ferroxidase, facilitating iron export from intestinal enterocytes into the bloodstream. This function is crucial for maintaining iron homeostasis, ensuring iron availability for essential biological processes, including hemoglobin synthesis and cellular respiration. Hephaestin operates in conjunction with ferroportin 1 to mediate iron transport across the basolateral membrane of enterocytes, linking iron and copper metabolism in mammals. Notably, copper deficiency can lead to decreased Hephaestin levels, resulting in impaired iron absorption and subsequent anemia. Hephaestin can bind up to six copper ions per monomer and is regulated by the homeobox transcription factor CDX2, with increased iron levels stimulating CDX2 expression and, consequently, Hephaestin production. While primarily expressed in the intestine, Hephaestin is also found in the colon, breast tissue, bone trabecular cells, and fibroblasts, highlighting diverse roles in various tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Hephaestin Antibody (C-7) References:
- Dietary copper deficiency reduces iron absorption and duodenal enterocyte hephaestin protein in male and female rats. | Reeves, PG., et al. 2005. J Nutr. 135: 92-8. PMID: 15623839
- Hephaestin--a ferroxidase of cellular iron export. | Petrak, J. and Vyoral, D. 2005. Int J Biochem Cell Biol. 37: 1173-8. PMID: 15778082
- CDX2-regulated expression of iron transport protein hephaestin in intestinal and colonic epithelium. | Hinoi, T., et al. 2005. Gastroenterology. 128: 946-61. PMID: 15825077
- Repletion of copper-deficient rats with dietary copper restores duodenal hephaestin protein and iron absorption. | Reeves, PG. and Demars, LC. 2005. Exp Biol Med (Maywood). 230: 320-5. PMID: 15855298
- Duodenal Dcytb and hephaestin mRNA expression are not significantly modulated by variations in body iron homeostasis. | Gleeson, F., et al. 2005. Blood Cells Mol Dis. 35: 303-8. PMID: 16137899
- Mechanisms of haem and non-haem iron absorption: lessons from inherited disorders of iron metabolism. | Anderson, GJ., et al. 2005. Biometals. 18: 339-48. PMID: 16158226
- Recent advances in intestinal iron transport. | Anderson, GJ. and Frazer, DM. 2005. Curr Gastroenterol Rep. 7: 365-72. PMID: 16168234
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hephaestin Antibody (C-7) | sc-365365 | 200 µg/ml | $322.00 | |||
Hephaestin Antibody (C-7): m-IgGκ BP-HRP Bundle | sc-522223 | 200 µg Ab, 40 µg BP | $361.00 | |||
Hephaestin Antibody (C-7): m-IgG2b BP-HRP Bundle | sc-549015 | 200 µg Ab; 10 µg BP | $361.00 | |||
Hephaestin Antibody (C-7) AC | sc-365365 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Hephaestin Antibody (C-7) HRP | sc-365365 HRP | 200 µg/ml | $322.00 | |||
Hephaestin Antibody (C-7) FITC | sc-365365 FITC | 200 µg/ml | $336.00 | |||
Hephaestin Antibody (C-7) PE | sc-365365 PE | 200 µg/ml | $349.00 | |||
Hephaestin Antibody (C-7) Alexa Fluor® 488 | sc-365365 AF488 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-7) Alexa Fluor® 546 | sc-365365 AF546 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-7) Alexa Fluor® 594 | sc-365365 AF594 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-7) Alexa Fluor® 647 | sc-365365 AF647 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-7) Alexa Fluor® 680 | sc-365365 AF680 | 200 µg/ml | $364.00 | |||
Hephaestin Antibody (C-7) Alexa Fluor® 790 | sc-365365 AF790 | 200 µg/ml | $364.00 |