Anti-Hec1 Antibody (C-11) is a mouse monoclonal IgG2b (kappa light chain) Hec1 antibody provided at 200 µg/ml
raised against amino acids 349-468 mapping within an internal region of Hec1 of human origin
Anti-Hec1 Antibody (C-11) is recommended for detection of Hec1 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-Hec1 Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Hec1 (C-11): sc-515550.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Hec1 Antibody (C-11) is a high quality monoclonal Hec1 antibody (also designated Hec1 antibody) suitable for the detection of the Hec1 protein of mouse, rat and human origin. Hec1 Antibody (C-11) is available as both the non-conjugated anti-Hec1 antibody form, as well as multiple conjugated forms of anti-Hec1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Highly expressed in cancer (Hec1) is a coiled-coil-enriched protein expressed abundantly in the S and M phases of rapidly dividing cells where it localizes to the kinetochores. Hec1 is involved in spindle checkpoint signaling. Hec1 is not expressed in terminal differentiated cells. Hec1 is expressed in tissues with high mitotic rates including testis, spleen and thymus. Hec1 is also found in the late S to M phases of bladder carcinoma cells. In dividing cells, Hec1 is required for the recruitment of Mps1 kinase and MAD1/MAD2 complexes to the kinetochores. The phosphorylation of Hec1 on Serine 165 by Nek2 is essential for faithful chromosome segregation. The binding of retinoblastoma protein to Hec1 also increases the fidelity of chromosomal segregation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What blocking solution should I use with Hec1 (C-11): sc-515550 monoclonal antibody for Western blot?
Asked by: Trav11
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
Rated 5 out of
Great job!I bought this a month ago. Using 1:500 can get a perfect results.
Date published: 2020-03-29
Rated 5 out of
Great antibody for WB!It works really well in WB. Dilution 1:3000. The cell extracts were from human colon carcinoma cells. It gives a strong band at 80 kDa.
Date published: 2017-10-19
Rated 5 out of
ImmunofluorescenceGreat staining in human skin fibroblasts and adrenocortical cells at a concentration of 1:50.
Date published: 2017-08-12
Rated 5 out of
做卵母细胞和卵母细胞铺片的免疫荧光特别好用之前申请的试用装，做卵母细胞和卵母细胞铺片的免疫荧光特别好用，想再买一支正装，具体使用方法是：卵母细胞的免疫荧光，固定透化20min. 1%BSA封闭一个小时，1:200使用Hec1，16h以上，二抗1:200 37°1h染的特别好。卵母细胞铺片的免疫荧光：和卵母细胞的免疫荧光使用条件一致，染的特别好，很特异，没有杂信号。
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