Hec1 Antibody (C-11) recommended for detection of Hec1 of mouse, rat and human origin by WB, IP, IF and ELISA

Hec1 Antibody (C-11): sc-515550

Hec1 Antibody (C-11) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-Hec1 Antibody (C-11) is a mouse monoclonal IgG2b (kappa light chain) Hec1 antibody provided at 200 µg/ml
    • raised against amino acids 349-468 mapping within an internal region of Hec1 of human origin
    • Anti-Hec1 Antibody (C-11) is recommended for detection of Hec1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Hec1 Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Hec1 (C-11): sc-515550.
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What blocking solution should I use with Hec1 (C-11): sc-515550 monoclonal antibody for Western blot?

Asked by: Trav11
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Great job! I bought this a month ago. Using 1:500 can get a perfect results.
Date published: 2020-03-29
Rated 5 out of 5 by from Great antibody for WB! It works really well in WB. Dilution 1:3000. The cell extracts were from human colon carcinoma cells. It gives a strong band at 80 kDa.
Date published: 2017-10-19
Rated 5 out of 5 by from Immunofluorescence Great staining in human skin fibroblasts and adrenocortical cells at a concentration of 1:50.
Date published: 2017-08-12
Rated 5 out of 5 by from 做卵母细胞和卵母细胞铺片的免疫荧光特别好用 之前申请的试用装,做卵母细胞和卵母细胞铺片的免疫荧光特别好用,想再买一支正装,具体使用方法是:卵母细胞的免疫荧光,固定透化20min. 1%BSA封闭一个小时,1:200使用Hec1,16h以上,二抗1:200 37°1h染的特别好。卵母细胞铺片的免疫荧光:和卵母细胞的免疫荧光使用条件一致,染的特别好,很特异,没有杂信号。
Date published: 2017-05-03
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