Date published: 2025-9-5
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Histone Deacetylase 5 (HDAC5) Antibody (B-11) - Western Blotting - Image 43019
HDAC5 抗体 (B-11): sc-133106. IMR-32 (A) 和 KNRK (B) 全细胞裂解液中 HDAC5 表达的 Western 印迹分析.
HDAC5 抗体 (B-11): sc-133106. IMR-32 (A) 和 KNRK (B) 全细胞裂解液中 HDAC5 表达的 Western 印迹分析.

Histone Deacetylase 5 (HDAC5) 抗体 (B-11): sc-133106

4.9(8)
寫評論提問

说明书
  • Histone Deacetylase 5 (HDAC5)抗体(B-11)是小鼠单克隆IgG2b κ, 在26篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HDAC5的氨基酸371-443
  • 抗-Histone Deacetylase 5 (HDAC5) 抗体 (B-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HDAC5
  • 抗Histone Deacetylase 5 (HDAC5)抗体（B-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Histone Deacetylase 5 (HDAC5) (B-11): sc-133106的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Histone Deacetylase 5 (HDAC5) 抗体 (B-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Histone Deacetylase 5 (HDAC5) 抗体 (B-11) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外HDAC抗体，包括HDAC1, HDAC10, HDAC11, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7和HDAC8

Histone Deacetylase 5 (HDAC5) 抗体（B-11）是一种 IgG2b κ 型小鼠单克隆 HDAC5 抗体，可通过WB, IP, IF, IHC(P)和ELISA检测小鼠、大鼠和人类来源的 HDAC5。Histone Deacetylase 5 (HDAC5) 抗体（B-11）既可作为非共轭的抗 HDAC5 抗体形式提供，也可作为多种共轭形式的抗 HDAC5 抗体提供，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 共轭物。在完整的细胞中，DNA 与组蛋白和其他核蛋白紧密结合形成染色质。人们认为染色质的重塑是转录调控的关键组成部分，而这种重塑的主要来源是核小体组蛋白的乙酰化。组蛋白氨基端尾部结构域的赖氨酸残基乙酰化导致核小体构象发生别构变化，并使 DNA 更容易被转录因子接近。相反，组蛋白的去乙酰化与转录沉默相关。已发现几种哺乳动物蛋白质为核组蛋白乙酰化酶，包括 GCN5、PCAF（p300/CBP 相关因子）、p300/CBP、HAT1 和 TFIID 亚基 TAF II p250。已确定哺乳动物 HDAC1（也称为 HD1）、HDAC2（也称为 RPD3）和 HDAC3-6 为组蛋白去乙酰化酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Histone Deacetylase 5 抗体 (B-11)

sc-133106
200 µg/ml
$316.00

Histone Deacetylase 5 (HDAC5) (B-11): m-IgGκ BP-HRP 套装

sc-521237
200 µg Ab, 40 µg BP
$354.00

Histone Deacetylase 5 (HDAC5) (B-11): m-IgG2b BP-HRP 套装

sc-548876
200 µg Ab; 10 µg BP
$354.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) AC

sc-133106 AC
500 µg/ml, 25% agarose
$416.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) HRP

sc-133106 HRP
200 µg/ml
$316.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) FITC

sc-133106 FITC
200 µg/ml
$330.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) PE

sc-133106 PE
200 µg/ml
$343.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) Alexa Fluor® 488

sc-133106 AF488
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) Alexa Fluor® 546

sc-133106 AF546
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) Alexa Fluor® 594

sc-133106 AF594
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) Alexa Fluor® 647

sc-133106 AF647
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) Alexa Fluor® 680

sc-133106 AF680
200 µg/ml
$357.00

Histone Deacetylase 5 (HDAC5) 抗体 (B-11) Alexa Fluor® 790

sc-133106 AF790
200 µg/ml
$357.00

Can this antibody be used on fixed mouse tissue in IF to detect HDAC5 via a secondary? Will there be too much background without a mouse on mouse block?

Asked by: pat68
Thank you for inquiring with us. This antibody can be used for detection of HDAC5 in mouse samples as long as the tissue isn't known to have high endogenous IgG levels. We recommend using our Mouse IgG Binding Proteins as secondaries (https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-130b1ry) to reduce non-specific staining. Mouse-on-mouse kits can also be used to reduce potential non-specific staining. Alternatively try our HDAC5 (C-11) antibody sc-133225 which is available conjugated to AF488, AF647, FITC, and PE.
Answered by: Technical Service
Date published: 2017-04-19

Can you recommend a positive control to use with HDAC5 (B-11): sc-133106 monoclonal antibody for Western blot?

Asked by: jenni
We recommend IMR-32 (sc-2409) or KNRK (sc-2214) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of HDAC5 in these cell lines.
Answered by: Technical Support 4
Date published: 2017-01-17
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Rated 4 out of 5 by from Also detects a smaller band < 25kDaUsing human blood monocyte derived macrophages, 25 ug total cell lysate ran on midi gels, 1:500 dilution in milk, 1:4000 2nd Ab CST anti-mouse we could detect a band at right size 10 minute exposure. But there is also a smaller band <25kda which did light up much faster and was stronger.
Date published: 2021-07-02
Rated 5 out of 5 by from band was niceWe used it in our WB and it worked well, HDAC5 gave best signal to background
Date published: 2017-04-30
Rated 5 out of 5 by from Great Antibody for WB, IHC(P), and IFHDAC5 (B-11) works great for WB, IHC(P), and IF. Nice clean band in positive control KNRK Whole Cell Lysate: sc-2214 at 140-150 kDa.
Date published: 2017-01-13
Rated 5 out of 5 by from One of the great antibody from SCBTOne of the great antibody from SCBT. completely validated in HDAC5 knockout mice. Used in Western Blot.
Date published: 2016-04-27
Rated 5 out of 5 by from Used for both WB and IP in human cellsUsed for both WB and IP in human cells- got correct band at 1:500 dilution.
Date published: 2015-10-28
Rated 5 out of 5 by from the antibody is very good and the bandsthe antibody is very good and the bands are clear .
Date published: 2015-04-14
Rated 5 out of 5 by from Positive western blot results in IMRPositive western blot results in IMR-32 and KNRK whole cell lysates.
Date published: 2015-03-17
Rated 5 out of 5 by from Cytoplasmic localization detected in immunofluorescenceCytoplasmic localization detected in immunofluorescence staining of formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-08
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Histone Deacetylase 5 (HDAC5) 抗体 (B-11) is rated 4.9 out of 5 by 8.
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