HDAC4 Antibody (A-4) recommended for detection of HDAC4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HDAC4 Antibody (A-4): sc-46672

HDAC4 Antibody (A-4) is rated 4.8 out of 5 by 10.
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Datasheets

    • Anti-HDAC4 Antibody (A-4) is a mouse monoclonal IgG2b (kappa light chain) HDAC4 antibody provided at 200 µg/ml
    • raised against amino acids 530-631 of HDAC4 of mouse origin
    • Anti-HDAC4 Antibody (A-4) is recommended for detection of HDAC4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-HDAC4 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-46672 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HDAC4 (A-4): sc-46672.

When should the TransCruz reagent sc-46672 X be used instead of the standard form sc-46672?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from 抗体一般 用了试用装,特异性还算比较强,但效价不高,需要上的浓度比较大,背景较为清晰,有需要应该会再次购买,谢谢
Date published: 2018-06-20
Rated 5 out of 5 by from Good quality! Work well in WB at 1:500.The band is quite clear.I incubated it at 4℃ for 14 hours.
Date published: 2017-12-13
Rated 5 out of 5 by from good quality! It work well in 1:500,transfer to the cellulose film took 1 and a half hours, the band is very clear.
Date published: 2017-12-13
Rated 5 out of 5 by from very good We bought this antibody last month, the concentration we used for WB is 1:500.
Date published: 2017-07-06
Rated 5 out of 5 by from Really strong antibody! Excellent choice for IHC on paraffin embedded tissue sections, and nice strong bands on Western blots. Solid antibody for HDAC4.
Date published: 2017-01-27
Rated 5 out of 5 by from Western blot and ChIP publication using T47D Western blot and ChIP publication using T47D cell samples -SCBT Publication Review
Date published: 2015-03-05
Rated 5 out of 5 by from Publication using human thyroid paraffin Publication using human thyroid paraffin-embedded tissue samples in IHC -SCBT Publication Review
Date published: 2015-02-19
Rated 5 out of 5 by from Western blot publication using 5637 Western blot publication using 5637, VmCub1, SW1710, UM-UC-3, T24, 639v, UP209 and UP210 samples -SCBT Publication Review
Date published: 2015-01-21
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