Date published: 2026-4-5

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GST Antibody (1E5): sc-53909

4.5(8)
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Datasheets
  • GST Antibody (1E5) is a mouse monoclonal IgG2b, cited in 33 publications, provided at 50 µg/500 µl
  • raised against recombinant Glutathione S-transferase
  • recommended for detection of GST by WB and IP
  • See GST (B-14): sc-138 for GST antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for GST Antibody (1E5) for WB applications. These reagents are now offered in bundles with GST Antibody (1E5) (see ordering information below).
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GST Antibody (1E5) is a mouse monoclonal IgG2b antibody that detects glutathione-S-transferase (GST) through applications such as western blotting (WB) and immunoprecipitation (IP). Anti-GST antibody (1E5) is available in a non-conjugated form, making GST (1E5) antibody an ideal choice for various experimental setups. Glutathione-S-transferase plays a crucial role in the detoxification of harmful compounds by catalyzing the conjugation of glutathione to a wide range of substrates, which is essential for cellular protection against oxidative stress and xenobiotic damage. This enzymatic function is particularly important in the liver, where GST helps to process and eliminate toxins, thereby maintaining metabolic homeostasis. The ability of GST to form fusion proteins with other proteins, as seen in the widely used pGEX.3X expression vector, allows researchers to study protein interactions and functions in a controlled manner. By utilizing GST fusion proteins, scientists can effectively monitor protein expression levels in various biological samples, making GST monoclonal antibody (1E5) a valuable tool for both basic and applied research in molecular biology and biochemistry.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

GST Antibody (1E5)

sc-53909
50 µg/500 µl
$322.00

GST Antibody (1E5): m-IgG Fc BP-HRP Bundle

sc-539012
50 µg Ab; 10 µg BP
$361.00

GST Antibody (1E5): m-IgG2b BP-HRP Bundle

sc-549725
50 µg Ab; 10 µg BP
$361.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 5 out of 5 by from Excellent for WBThe (1E5) clone is well established and a popular choice for detection of GST fusion proteins in WB.
Date published: 2017-01-07
Rated 5 out of 5 by from Helpful!I used this GST antibody in my WB assay and recieved excellent result. It can indentify my GST tagged fusion protein specifically. So I think its a good product.
Date published: 2016-12-31
Rated 5 out of 5 by from GST antibody in WBI have already used this GST antibody in my WB assay and recieved excellent result. It can indentify my GST tagged fusion protein specifically.
Date published: 2016-11-24
Rated 1 out of 5 by from Does not work in Western BlotDoes not work in Western Blot.
Date published: 2015-03-31
Rated 5 out of 5 by from Good results with PCGood results with PC-3, DU-145 and LNCaP cell lines in WB -SCBT Publication Review
Date published: 2015-02-19
Rated 5 out of 5 by from Used for WB with PCUsed for WB with PC-3, MDCK and COS-7 cell lines -SCBT Publication Review
Date published: 2014-01-09
Rated 5 out of 5 by from Detects recombinant GST and GSTDetects recombinant GST and GST-tagged proteins in WB -SCBT QC
Date published: 2013-12-13
Rated 5 out of 5 by from Worked in WB with transfected 293T cellsWorked in WB with transfected 293T cells -SCBT Publication Review
Date published: 2013-05-21
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GST Antibody (1E5) is rated 4.5 out of 5 by 8.
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