GST Antibody (B-14): sc-138

GST Antibody (B-14) is a mouse monoclonal IgG1 kappa light chain antibody that detects Glutathione S-Transferase (GST) from Schistosoma japonicum by western blotting (WB) and immunoprecipitation (IP). Anti-GST antibody (B-14) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. GST plays a crucial role in detoxifying harmful compounds by catalyzing the conjugation of glutathione to various substrates, which is essential for cellular protection against oxidative stress and xenobiotic damage. This function is particularly important in Schistosoma japonicum, as GST helps the parasite survive in the host environment by neutralizing potentially harmful substances. Additionally, plasmid vectors such as pGEX.3X are commonly used to express hybrid fusion proteins that include GST, facilitating the study of protein interactions and functions. GST (B-14) antibody is invaluable for verifying protein expression in both plaques and western blotting (WB), as well as for the immunoaffinity purification of proteins expressed in Escherichia coli, making GST (B-14) monoclonal antibody an essential tool for researchers in the field.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GST Antibody (B-14) References:

1. Expression of GST-fused kinase domain of human Csk homologous kinase from Pichia pastoris facilitates easy purification.  |  Murthy, TV. 2004. Biotechnol Lett. 26: 443-9. PMID: 15104145
2. Glutathione-S-transferase-pi (GST-pi) expression in renal cell carcinoma.  |  Kaprilian, C., et al. 2015. J Kidney Cancer VHL. 2: 25-29. PMID: 28326256
3. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.  |  Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
4. Glutathione-S-transferase (GST) catalyzes the degradation of Chlorimuron-ethyl by Klebsiella jilinsis 2N3.  |  Zhang, S., et al. 2020. Sci Total Environ. 729: 139075. PMID: 32388135
5. Detection of Protein-Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique.  |  Gnanasekaran, P. and Pappu, HR. 2023. Methods Mol Biol. 2690: 111-115. PMID: 37450141
6. Polymorphisms in Glutathione S-Transferase (GST) Genes Modify the Effect of Exposure to Maternal Smoking Metabolites in Pregnancy and Offspring DNA Methylation.  |  Kheirkhah Rahimabad, P., et al. 2023. Genes (Basel). 14: PMID: 37628696
7. Cloning, gene sequencing, and expression of the small molecular mass ubiquinone-binding protein of mitochondrial ubiquinol-cytochrome c reductase.  |  Yu, L., et al. 1995. J Biol Chem. 270: 25634-8. PMID: 7592738
8. Expression of small regions of equine herpesvirus 1 glycoprotein C in Escherichia coli.  |  Crabb, BS. and Studdert, MJ. 1995. Vet Microbiol. 46: 181-91. PMID: 8545955
9. Assessment of antigenicity and genetic variation of glycoprotein B of murine cytomegalovirus.  |  Xu, J., et al. 1996. J Gen Virol. 77 (Pt 1): 49-59. PMID: 8558128
10. Matrilysin: expression, purification, and characterization.  |  Soler, D., et al. 1995. J Protein Chem. 14: 511-20. PMID: 8561847
11. Functional expression of subunit IV of Rhodobacter sphaeroides cytochrome b-c1 complex and reconstitution of recombinant protein with three-subunit core complex.  |  Chen, YR., et al. 1996. J Biol Chem. 271: 2057-62. PMID: 8567659
12. Functional comparison of native and recombinant human salivary histatin 1.  |  Driscoll, J., et al. 1995. J Dent Res. 74: 1837-44. PMID: 8600179

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GST Antibody (B-14) has 1,414 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GST Antibody (B-14) Specifications

Alternate Product Name: GST

Product Clone Name: B-14

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: GST

Epitope Species:

Product Reactivity/Detection: GST fusion proteins and glutathione-S-transferase (GST)

Species Reactivity/Detection: Schistosoma japonicum

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1,414 citations in various scientific publications

Available Conjugates/Forms for GST Antibody (B-14): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine