GSK3 beta Antibody (E-11): sc-377213

GSK-3β Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to detect glycogen synthase kinase 3 beta (GSK-3β) across mouse, rat, and human species through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-GSK-3β antibody (E-11) targets the C-terminal epitope comprising amino acids 345-420 of human GSK-3β, ensuring precise binding and reliable detection. GSK-3β, also referred to as GSK3, GSK-3, and GSK-3beta, is a critical serine/threonine kinase involved in cellular processes, including glycogen metabolism, cell cycle regulation, and various signaling pathways like Wnt and insulin pathways. GSK-3β interacts with several key proteins such as beta-catenin, where GSK-3β phosphorylates and regulates beta-catenin′s stability, and tau protein, whose hyperphosphorylation by GSK-3β is a hallmark of Alzheimer′s disease pathology. Additionally, GSK-3β interacts with proteins like Akt, which phosphorylates and inhibits activity, and Axin and APC, which are components of the Wnt signaling pathway that modulate function. Anti-GSK-3β antibody (E-11) is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing flexibility for diverse experimental needs. GSK-3β monoclonal antibody (E-11) facilitates the study of disease mechanisms, particularly in neurodegenerative conditions and cancer, where dysregulation of GSK-3β signaling plays a pivotal role. Anti-GSK-3β antibody (E-11) offers robust performance and specificity to support research investigating protein-protein interactions, signaling pathways, or disease models.

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GSK3 beta Antibody (E-11) References:

1. The Role of GSK-3β in the Regulation of Protein Turnover, Myosin Phenotype, and Oxidative Capacity in Skeletal Muscle under Disuse Conditions.  |  Mirzoev, TM., et al. 2021. Int J Mol Sci. 22: PMID: 34064895
2. Cardiomyocyte-GSK-3β deficiency induces cardiac progenitor cell proliferation in the ischemic heart through paracrine mechanisms.  |  Yusuf, AM., et al. 2022. J Cell Physiol. 237: 1804-1817. PMID: 34812500
3. PACT inhibits the replication of SARS-CoV-2 through the blockage of GSK-3β-N-nsp3 cascade.  |  Lin, YY., et al. 2023. J Med Virol. 95: e28832. PMID: 37264691
4. Dual-target inhibitors of cholinesterase and GSK-3β to modulate Alzheimer's disease.  |  He, J. and Tam, KY. 2024. Drug Discov Today. 29: 103914. PMID: 38340951
5. Networking with proline-directed protein kinases implicated in tau phosphorylation.  |  Pelech, SL. 1995. Neurobiol Aging. 16: 247-56; discussion 257-61. PMID: 7566335
6. Glycogen synthase kinase-3 beta phosphorylates tau protein at multiple sites in intact cells.  |  Sperber, BR., et al. 1995. Neurosci Lett. 197: 149-53. PMID: 8552282
7. Regulation of glycogen synthase activation in isolated hepatocytes.  |  Pugazhenthi, S. and Khandelwal, RL. 1995. Mol Cell Biochem. 149-150: 95-101. PMID: 8569754
8. Different localization of tau protein kinase I/glycogen synthase kinase-3 beta from glycogen synthase kinase-3 alpha in cerebellum mitochondria.  |  Hoshi, M., et al. 1995. J Biochem. 118: 683-5. PMID: 8576078
9. Regulation of mitochondrial pyruvate dehydrogenase activity by tau protein kinase I/glycogen synthase kinase 3beta in brain.  |  Hoshi, M., et al. 1996. Proc Natl Acad Sci U S A. 93: 2719-23. PMID: 8610107
10. Binding of GSK3beta to the APC-beta-catenin complex and regulation of complex assembly.  |  Rubinfeld, B., et al. 1996. Science. 272: 1023-6. PMID: 8638126
11. Differential phosphorylation of human tau isoforms containing three repeats by several protein kinases.  |  Singh, TJ., et al. 1996. Arch Biochem Biophys. 328: 43-50. PMID: 8638936
12. Tau is enriched on dynamic microtubules in the distal region of growing axons.  |  Black, MM., et al. 1996. J Neurosci. 16: 3601-19. PMID: 8642405

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GSK3 beta Antibody (E-11) has 67 citations in a variety of scientific publications.

Primary antibodies like Anti-GSK3 beta Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GSK3 beta Antibody (E-11) Specifications

Alternate Product Name: GSK3

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 345-420

Epitope Target: GSK-3β

Epitope Species: human

Product Reactivity/Detection: GSK-3β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 67 citations in various scientific publications

Available Conjugates/Forms for GSK3 beta Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred GSK3 alpha/beta antibody is GSK3 alpha/beta Antibody (0011-A): sc-7291.