granzyme A Antibody (3G8.5): sc-33692

granzyme A Antibody (3G8.5) is a mouse monoclonal IgG2b kappa light chain antibody that detects granzyme A protein of mouse origin by western blotting (WB), immunoprecipitation (IP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). anti-granzyme A antibody (3G8.5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Granzyme A plays a crucial role in the immune response, particularly in the induction of apoptosis in target cells, which is essential for maintaining tissue homeostasis and eliminating infected or malignant cells. This serine protease is primarily located within cytolytic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells, where granzyme A is stored as an inactive proenzyme until released during the degranulation process. Upon activation, granzyme A induces apoptosis through a unique pathway that involves the induction of single-strand DNA breaks and membrane perturbation, distinguishing its mechanism from that of granzyme B, which primarily activates caspases. The ability of granzyme A to function independently of caspase activation highlights its importance in the immune system′s ability to eliminate cells that pose a threat, making granzyme A a vital target for research in immunology and cancer therapy.

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granzyme A Antibody (3G8.5) References:

1. Granzyme A loading induces rapid cytolysis and a novel form of DNA damage independently of caspase activation.  |  Beresford, PJ., et al. 1999. Immunity. 10: 585-94. PMID: 10367904
2. Granzyme A initiates an alternative pathway for granule-mediated apoptosis.  |  Shresta, S., et al. 1999. Immunity. 10: 595-605. PMID: 10367905
3. Dipeptidyl peptidase I is required for the processing and activation of granzymes A and B in vivo.  |  Pham, CT. and Ley, TJ. 1999. Proc Natl Acad Sci U S A. 96: 8627-32. PMID: 10411926
4. Granzyme A as a potential biomarker of Mycobacterium tuberculosis infection and disease.  |  Guggino, G., et al. 2015. Immunol Lett. 166: 87-91. PMID: 26051682
5. Granzyme A in Chikungunya and Other Arboviral Infections.  |  Schanoski, AS., et al. 2019. Front Immunol. 10: 3083. PMID: 31993061
6. Granzyme A from cytotoxic lymphocytes cleaves GSDMB to trigger pyroptosis in target cells.  |  Zhou, Z., et al. 2020. Science. 368: PMID: 32299851
7. Modulation of Inflammation by Extracellular Granzyme A.  |  van Daalen, KR., et al. 2020. Front Immunol. 11: 931. PMID: 32508827
8. Cloning and chromosomal assignment of a human cDNA encoding a T cell- and natural killer cell-specific trypsin-like serine protease.  |  Gershenfeld, HK., et al. 1988. Proc Natl Acad Sci U S A. 85: 1184-8. PMID: 3257574
9. Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells.  |  Shresta, S., et al. 1995. Proc Natl Acad Sci U S A. 92: 5679-83. PMID: 7777569
10. Localization of granzyme B in the nucleus. A putative role in the mechanism of cytotoxic lymphocyte-mediated apoptosis.  |  Trapani, JA., et al. 1996. J Biol Chem. 271: 4127-33. PMID: 8626751
11. Cytotoxic T lymphocyte-assisted suicide. Caspase 3 activation is primarily the result of the direct action of granzyme B.  |  Atkinson, EA., et al. 1998. J Biol Chem. 273: 21261-6. PMID: 9694885
12. Efficient nuclear targeting of granzyme B and the nuclear consequences of apoptosis induced by granzyme B and perforin are caspase-dependent, but cell death is caspase-independent.  |  Trapani, JA., et al. 1998. J Biol Chem. 273: 27934-8. PMID: 9774406

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-granzyme A Antibody (3G8.5) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-granzyme A Antibody (3G8.5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

granzyme A Antibody (3G8.5) Specifications

Alternate Product Name: granzyme A

Product Clone Name: 3G8.5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-262

Epitope Target: granzyme A

Epitope Species: mouse

Product Reactivity/Detection: granzyme A

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for granzyme A Antibody (3G8.5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin