GlyR β Antibody (D-8): sc-365819
- GlyR β Antibody (D-8) is a mouse monoclonal IgG1 κ GlyR β antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 412-441 near the C-terminus of GlyR β of human origin
- GlyR beta Antibody (D-8) is recommended for detection of GlyR β of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GlyR beta Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GlyR β Antibody (D-8) for WB applications. These reagents are now offered in bundles with GlyR β Antibody (D-8) (see ordering information below).
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GlyR β Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects GlyR beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GlyR β (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GlyR beta protein plays a crucial role in the central nervous system by mediating inhibitory neurotransmission through glycine receptors, which are essential for voluntary motor control and reflex responses. Glycine binds to these receptors located in the postsynaptic neuronal membranes, allowing for the regulation of chloride ion flow, which is vital for maintaining the balance of excitatory and inhibitory signals in the brain. The pentameric structure of GlyR consists of various subunits, including α1 through α4 and β, which together form a chloride-selective ion channel. The composition of these subunits shifts from α2 in the fetal central nervous system to α1 and β in adults, highlighting GlyR beta′s developmental significance. Fast potentiation of GlyR by intracellular calcium ions in regions such as the brainstem and midbrain underscores the importance of calcium in modulating glycinergic synapses, further emphasizing GlyR beta protein′s critical role in neurotransmission and synaptic plasticity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GlyR β Antibody (D-8) References:
- An update on GABAA receptors. | Mehta, AK. and Ticku, MK. 1999. Brain Res Brain Res Rev. 29: 196-217. PMID: 10209232
- Fast potentiation of glycine receptor channels of intracellular calcium in neurons and transfected cells. | Fucile, S., et al. 2000. Neuron. 28: 571-83. PMID: 11144365
- Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genes. | Grenningloh, G., et al. 1990. EMBO J. 9: 771-6. PMID: 2155780
- Conserved quaternary structure of ligand-gated ion channels: the postsynaptic glycine receptor is a pentamer. | Langosch, D., et al. 1988. Proc Natl Acad Sci U S A. 85: 7394-8. PMID: 2459705
- Primary cultures of mouse spinal cord express the neonatal isoform of the inhibitory glycine receptor. | Hoch, W., et al. 1989. Neuron. 3: 339-48. PMID: 2561971
- The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors. | Grenningloh, G., et al. Nature. 328: 215-20. PMID: 3037383
- Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family. | Schofield, PR., et al. Nature. 328: 221-7. PMID: 3037384
- Solubilization of the glycine receptor from rat spinal cord. | Pfeiffer, F. and Betz, H. 1981. Brain Res. 226: 273-9. PMID: 6271346
- Purification by affinity chromatography of the glycine receptor of rat spinal cord. | Pfeiffer, F., et al. 1982. J Biol Chem. 257: 9389-93. PMID: 6286620
- The human glycine receptor beta subunit gene (GLRB): structure, refined chromosomal localization, and population polymorphism. | Milani, N., et al. 1998. Genomics. 50: 341-5. PMID: 9676428
- The human glycine receptor subunit alpha3. Glra3 gene structure, chromosomal localization, and functional characterization of alternative transcripts. | Nikolic, Z., et al. 1998. J Biol Chem. 273: 19708-14. PMID: 9677400
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GlyR β Antibody (D-8) | sc-365819 | 200 µg/ml | $322.00 | |||
GlyR β Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-527397 | 200 µg Ab; 10 µg BP | $361.00 | |||
GlyR β Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-532770 | 200 µg Ab; 20 µg BP | $361.00 | |||
GlyR β Antibody (D-8) AC | sc-365819 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GlyR β Antibody (D-8) HRP | sc-365819 HRP | 200 µg/ml | $322.00 | |||
GlyR β Antibody (D-8) FITC | sc-365819 FITC | 200 µg/ml | $336.00 | |||
GlyR β Antibody (D-8) PE | sc-365819 PE | 200 µg/ml | $349.00 | |||
GlyR β Antibody (D-8) Alexa Fluor® 488 | sc-365819 AF488 | 200 µg/ml | $364.00 | |||
GlyR β Antibody (D-8) Alexa Fluor® 546 | sc-365819 AF546 | 200 µg/ml | $364.00 | |||
GlyR β Antibody (D-8) Alexa Fluor® 594 | sc-365819 AF594 | 200 µg/ml | $364.00 | |||
GlyR β Antibody (D-8) Alexa Fluor® 647 | sc-365819 AF647 | 200 µg/ml | $364.00 | |||
GlyR β Antibody (D-8) Alexa Fluor® 680 | sc-365819 AF680 | 200 µg/ml | $364.00 | |||
GlyR β Antibody (D-8) Alexa Fluor® 790 | sc-365819 AF790 | 200 µg/ml | $364.00 | |||
GlyR β (D-8) Neutralizing Peptide | sc-365819 P | 100 µg/0.5 ml | $69.00 |