GlyR α2 Antibody (C-11): sc-398964

GlyR α2 Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects GlyR alpha 2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-GlyR α2 antibody (C-11) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GlyR alpha 2 protein plays a crucial role in the central nervous system by mediating inhibitory neurotransmission through glycine receptors, which are essential for voluntary motor control and reflex responses. Glycine binds to these receptors located in postsynaptic neuronal membranes, allowing for chloride ion flow regulation, vital for maintaining the balance of excitatory and inhibitory signals in the brain. The pentameric structure of GlyR consists of various subunits, including α1 through α4 and β, which together form a chloride-selective ion channel. The composition of these subunits shifts from predominantly α2 in the fetal central nervous system to a mix of α1 and β subunits in adults, highlighting the dynamic nature of glycine receptor function during development. Fast potentiation of GlyR by intracellular calcium ions in regions such as the brainstem and midbrain underscores calcium′s importance in modulating glycinergic synapses, further emphasizing GlyR′s critical role in neurotransmission. The genes encoding human GlyR subunits are mapped to specific chromosomes, with α1, α2, α3, and β subunits located on chromosomes 5q32, Xp22, 4q33, and 4q31, respectively, providing insight into their genetic regulation and potential implications in neurological disorders.

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GlyR α2 Antibody (C-11) References:

1. An update on GABAA receptors.  |  Mehta, AK. and Ticku, MK. 1999. Brain Res Brain Res Rev. 29: 196-217. PMID: 10209232
2. Fast potentiation of glycine receptor channels of intracellular calcium in neurons and transfected cells.  |  Fucile, S., et al. 2000. Neuron. 28: 571-83. PMID: 11144365
3. Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genes.  |  Grenningloh, G., et al. 1990. EMBO J. 9: 771-6. PMID: 2155780
4. Conserved quaternary structure of ligand-gated ion channels: the postsynaptic glycine receptor is a pentamer.  |  Langosch, D., et al. 1988. Proc Natl Acad Sci U S A. 85: 7394-8. PMID: 2459705
5. Primary cultures of mouse spinal cord express the neonatal isoform of the inhibitory glycine receptor.  |  Hoch, W., et al. 1989. Neuron. 3: 339-48. PMID: 2561971
6. The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors.  |  Grenningloh, G., et al. Nature. 328: 215-20. PMID: 3037383
7. Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family.  |  Schofield, PR., et al. Nature. 328: 221-7. PMID: 3037384
8. Solubilization of the glycine receptor from rat spinal cord.  |  Pfeiffer, F. and Betz, H. 1981. Brain Res. 226: 273-9. PMID: 6271346
9. Purification by affinity chromatography of the glycine receptor of rat spinal cord.  |  Pfeiffer, F., et al. 1982. J Biol Chem. 257: 9389-93. PMID: 6286620
10. The human glycine receptor beta subunit gene (GLRB): structure, refined chromosomal localization, and population polymorphism.  |  Milani, N., et al. 1998. Genomics. 50: 341-5. PMID: 9676428
11. The human glycine receptor subunit alpha3. Glra3 gene structure, chromosomal localization, and functional characterization of alternative transcripts.  |  Nikolic, Z., et al. 1998. J Biol Chem. 273: 19708-14. PMID: 9677400

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GlyR α2 Antibody (C-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GlyR α2 Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GlyR α2 Antibody (C-11) Specifications

Alternate Product Name: GlyR α2

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 371-420

Epitope Target: GlyR α2

Epitope Species: human

Product Reactivity/Detection: GlyR α2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GlyR α2 Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin