Glucosidase IIα Antibody (D-1): sc-518226
- Glucosidase IIα Antibody (D-1) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 904-925 of Glucosidase IIα of human origin
- recommended for detection of Glucosidase IIα of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Glucosidase II alpha Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Glucosidase IIα Antibody (D-1) for WB applications. These reagents are now offered in bundles with Glucosidase IIα Antibody (D-1) (see ordering information below).
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Glucosidase IIα Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Glucosidase IIα in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Glucosidase IIα Antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glucosidase IIα plays a crucial role in processing N-linked glycoproteins by trimming glucose residues in the endoplasmic reticulum, which is essential for proper protein folding and quality control. Glucosidase IIα operates as part of a heterodimeric complex with Glucosidase IIβ, where Glucosidase IIα serves as the catalytic core, capable of functioning independently of its β counterpart. The interaction between these subunits is vital for enzymatic stability and activity, as Glucosidase IIβ contains a retention signal that ensures the complex remains within the endoplasmic reticulum, thereby facilitating efficient glycoprotein processing. The phosphorylated form of Glucosidase IIβ, highly expressed in fibroblasts and epidermal carcinoma cells, highlights Glucosidase IIα′s significance in cellular signaling pathways and potential role in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Glucosidase IIα Antibody (D-1) References:
- The alpha- and beta-subunits are required for expression of catalytic activity in the hetero-dimeric glucosidase II complex from human liver. | Treml, K., et al. 2000. Glycobiology. 10: 493-502. PMID: 10764838
- Quaternary and domain structure of glycoprotein processing glucosidase II. | Trombetta, ES., et al. 2001. Biochemistry. 40: 10717-22. PMID: 11524018
- Purification and characterization of glucosidase II involved in N-linked glycoprotein processing in bovine mammary gland. | Saxena, S., et al. 1987. Biochem J. 247: 563-70. PMID: 3122729
- Purification and characterization of glucosidase I involved in N-linked glycoprotein processing in bovine mammary gland. | Shailubhai, K., et al. 1987. Biochem J. 247: 555-62. PMID: 3322267
- Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunit. | Trombetta, ES., et al. 1996. J Biol Chem. 271: 27509-16. PMID: 8910335
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glucosidase IIα Antibody (D-1) | sc-518226 | 200 µg/ml | $316.00 | |||
Glucosidase IIα Antibody (D-1): m-IgG Fc BP-HRP Bundle | sc-551459 | 200 µg Ab; 10 µg BP | $354.00 | |||
Glucosidase IIα Antibody (D-1): m-IgG1 BP-HRP Bundle | sc-551458 | 200 µg Ab; 20 µg BP | $354.00 | |||
Glucosidase IIα Antibody (D-1): m-IgGκ BP-HRP Bundle | sc-551460 | 200 µg Ab; 40 µg BP | $354.00 | |||
Glucosidase IIα Antibody (D-1) AC | sc-518226 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Glucosidase IIα Antibody (D-1) HRP | sc-518226 HRP | 200 µg/ml | $316.00 | |||
Glucosidase IIα Antibody (D-1) FITC | sc-518226 FITC | 200 µg/ml | $330.00 | |||
Glucosidase IIα Antibody (D-1) PE | sc-518226 PE | 200 µg/ml | $343.00 | |||
Glucosidase IIα Antibody (D-1) Alexa Fluor® 488 | sc-518226 AF488 | 200 µg/ml | $357.00 | |||
Glucosidase IIα Antibody (D-1) Alexa Fluor® 546 | sc-518226 AF546 | 200 µg/ml | $357.00 | |||
Glucosidase IIα Antibody (D-1) Alexa Fluor® 594 | sc-518226 AF594 | 200 µg/ml | $357.00 | |||
Glucosidase IIα Antibody (D-1) Alexa Fluor® 647 | sc-518226 AF647 | 200 µg/ml | $357.00 | |||
Glucosidase IIα Antibody (D-1) Alexa Fluor® 680 | sc-518226 AF680 | 200 µg/ml | $357.00 | |||
Glucosidase IIα Antibody (D-1) Alexa Fluor® 790 | sc-518226 AF790 | 200 µg/ml | $357.00 |