GLI-3 Antibody (B-4): sc-74478

GLI-3 Antibody (B-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects GLI-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GLI-3 (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GLI-3, also known as GLI3FL, is a crucial regulator in the Sonic hedgehog (Shh) signaling pathway, functioning as both a transcriptional activator and repressor. This dual role is vital for proper embryonic development, particularly in the formation of limbs and craniofacial structures. GLI-3 is a member of the GLI family of zinc-finger proteins, characterized by C2H2-type zinc-finger domains that facilitate DNA binding and transcriptional regulation. GLI-3 is predominantly located in the nucleus and cytoplasm, where GLI-3 interacts with various transcriptional co-factors to modulate gene expression. Mutations in GLI-3 can lead to developmental disorders such as Greig cephalo-poly-syndactyly syndrome (GCPS), highlighting GLI-3′s significance in normal developmental processes.

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GLI-3 Antibody (B-4) References:

1. Point mutations throughout the GLI3 gene cause Greig cephalopolysyndactyly syndrome.  |  Kalff-Suske, M., et al. 1999. Hum Mol Genet. 8: 1769-77. PMID: 10441342
2. Hedgehog-regulated processing of Gli3 produces an anterior/posterior repressor gradient in the developing vertebrate limb.  |  Wang, B., et al. 2000. Cell. 100: 423-34. PMID: 10693759
3. Physical and functional interactions between Zic and Gli proteins.  |  Koyabu, Y., et al. 2001. J Biol Chem. 276: 6889-92. PMID: 11238441
4. [Progress in the study of KRAB zinc finger protein].  |  Tian, CY., et al. 2006. Yi Chuan. 28: 1451-6. PMID: 17098717
5. Characterization of the interactions of human ZIC3 mutants with GLI3.  |  Zhu, L., et al. 2008. Hum Mutat. 29: 99-105. PMID: 17764085
6. GLI3 encodes a 190-kilodalton protein with multiple regions of GLI similarity.  |  Ruppert, JM., et al. 1990. Mol Cell Biol. 10: 5408-15. PMID: 2118997
7. The GLI-Kruppel family of human genes.  |  Ruppert, JM., et al. 1988. Mol Cell Biol. 8: 3104-13. PMID: 2850480
8. An intron variant of the GLI family zinc finger 3 (GLI3) gene differentiates resistance training-induced muscle fiber hypertrophy in younger men.  |  Vann, CG., et al. 2021. FASEB J. 35: e21587. PMID: 33891350
9. GLI3 regulates muscle stem cell entry into GAlert and self-renewal.  |  Brun, CE., et al. 2022. Nat Commun. 13: 3961. PMID: 35803939
10. Identification of truncated variants in GLI family zinc finger 3 (GLI3) associated with polydactyly.  |  Wang, RY., et al. 2024. J Orthop Surg Res. 19: 449. PMID: 39080720
11. Point mutations in human GLI3 cause Greig syndrome.  |  Wild, A., et al. 1997. Hum Mol Genet. 6: 1979-84. PMID: 9302279

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GLI-3 Antibody (B-4) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-GLI-3 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GLI-3 Antibody (B-4) Specifications

Alternate Product Name: GLI3

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-280

Epitope Target: GLI-3

Epitope Species: human

Product Reactivity/Detection: GLI-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for GLI-3 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent