GLI-1/GLI1 Antibody (C-1): sc-515751

GLI-1 Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody engineered to recognize GLI-1, also known as Glioma-associated oncogene or oncogene GLI, from human origin. Anti-GLI-1 antibody (C-1) is raised against amino acids 781-1080 of the full-length GLI-1 protein, enabling effective detection in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GLI-1 is a member of the GLI family of zinc finger transcription factors and plays a pivotal role in the Hedgehog signaling pathway, which is critical for embryonic development, tissue patterning, and the maintenance of stem cell populations. Located primarily in the nucleus, GLI-1 functions as a transcriptional activator by binding to specific DNA sequences and regulating the expression of target genes involved in cell proliferation, differentiation, and survival. Nuclear localization of GLI-1 is essential for modulating gene expression, and dysregulation is implicated in various cancers, including gliomas, highlighting GLI-1′s importance in both normal physiology and oncogenic processes. GLI-1 interacts with other proteins such as SUFU and various co-activators to finely tune transcriptional activity, ensuring precise control of cellular responses to developmental and environmental cues. Anti-GLI-1 antibody (C-1) is an indispensable tool for researchers studying the mechanisms of Hedgehog signaling, cancer biology, and developmental biology, providing valuable insights into the regulation and function of this critical transcription factor. Available in both unconjugated and conjugated forms, GLI-1 monoclonal antibody (C-1) offers versatility for a wide range of experimental applications, facilitating advanced studies into the molecular pathways governed by GLI-1.

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GLI-1/GLI1 Antibody (C-1) References:

1. Gli regulation by the opposing activities of fused and suppressor of fused.  |  Murone, M., et al. 2000. Nat Cell Biol. 2: 310-2. PMID: 10806483
2. Letters to the editor: identification of polymorphic variants of the GLI1 oncogene.  |  Wang, XQ., et al. 2000. J Invest Dermatol. 115: 328-9. PMID: 10951255
3. Physical and functional interactions between Zic and Gli proteins.  |  Koyabu, Y., et al. 2001. J Biol Chem. 276: 6889-92. PMID: 11238441
4. A family of human zinc finger proteins that bind methylated DNA and repress transcription.  |  Filion, GJ., et al. 2006. Mol Cell Biol. 26: 169-81. PMID: 16354688
5. A comprehensive catalog of human KRAB-associated zinc finger genes: insights into the evolutionary history of a large family of transcriptional repressors.  |  Huntley, S., et al. 2006. Genome Res. 16: 669-77. PMID: 16606702
6. [Progress in the study of KRAB zinc finger protein].  |  Tian, CY., et al. 2006. Yi Chuan. 28: 1451-6. PMID: 17098717
7. Gli-1 siRNA induced apoptosis in Huh7 cells.  |  Chen, XL., et al. 2008. World J Gastroenterol. 14: 582-9. PMID: 18203291
8. A novel splice variant of GLI1 that promotes glioblastoma cell migration and invasion.  |  Lo, HW., et al. 2009. Cancer Res. 69: 6790-8. PMID: 19706761
9. The GLI gene is a member of the Kruppel family of zinc finger proteins.  |  Kinzler, KW., et al. 1988. Nature. 332: 371-4. PMID: 2832761
10. Immunohistochemical analysis of SHH, SMO and GLI-1 proteins in epithelial odontogenic lesions.  |  Rodrigues, KS., et al. 2022. Braz Dent J. 33: 91-99. PMID: 36287504
11. Icariin promotes the proliferation and osteogenic differentiation of bone-derived mesenchymal stem cells in patients with osteoporosis and T2DM by upregulating GLI-1.  |  Xia, SL., et al. 2023. J Orthop Surg Res. 18: 500. PMID: 37454090
12. Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers.  |  Pavletich, NP. and Pabo, CO. 1993. Science. 261: 1701-7. PMID: 8378770

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GLI-1/GLI1 Antibody (C-1) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-GLI-1/GLI1 Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GLI-1/GLI1 Antibody (C-1) Specifications

Alternate Product Name: GLI-1

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 781-1080

Epitope Target: GLI-1

Epitope Species: human

Product Reactivity/Detection: GLI-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for GLI-1/GLI1 Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent