GIT1 Antibody (E-7) recommended for detection of GIT1 of mouse, rat and human origin by WB, IP, IF and ELISA

GIT1 Antibody (E-7): sc-398637

GIT1 Antibody (E-7) is rated 5.0 out of 5 by 2.
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Datasheets

    • GIT1 Antibody (E-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 471-640 mapping near the N-terminus of GIT1 of human origin
    • recommended for detection of GIT1 of mouse, rat and human origin by WB, IP, IF and ELISA

What is the recommended fixation method for immunofluorescence staining with GIT1 (E-7): sc-398637 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced exceptional immunofluorescence focal Produced exceptional immunofluorescence focal adhesions, cytoplasmic and membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-19
Rated 5 out of 5 by from Nice Western Blot data of GIT1 expression Nice Western Blot data of GIT1 expression in human testis tissue extract and IMR-32 and HeLa whole cell lysates. -SCBT QC
Date published: 2013-01-16
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