GIT1 Antibody (E-7): sc-398637
- GIT1 Antibody (E-7) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 471-640 mapping near the N-terminus of GIT1 of human origin
- recommended for detection of GIT1 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GIT1 Antibody (E-7) for WB applications. These reagents are now offered in bundles with GIT1 Antibody (E-7) (see ordering information below).
QUICK LINKS
GIT1 Antibody (E-7) is a mouse monoclonal IgG2a antibody that detects GIT1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GIT1 plays a crucial role in the regulation of G protein-coupled receptor signaling by acting as a GTPase-activating protein for members of the ADP ribosylation factor family, which are essential for vesicular trafficking. This function is particularly important as GIT1 helps modulate the internalization and resensitization of receptors, such as the β2-adrenergic receptor, thereby influencing cellular responses to external signals. By phosphorylating and inactivating these receptors, GIT1 ensures that signaling pathways remain finely tuned, preventing overstimulation and maintaining cellular homeostasis. Anti-GIT1 antibody (E-7) serves as an invaluable tool for researchers studying the dynamics of G protein signaling and its implications in various physiological and pathological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GIT1 Antibody (E-7) References:
- GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. | Manabe, R., et al. 2002. J Cell Sci. 115: 1497-510. PMID: 11896197
- Characterization of the endogenous GIT1-betaPIX complex, and identification of its association to membranes. | Botrugno, OA., et al. 2006. Eur J Cell Biol. 85: 35-46. PMID: 16373173
- Identification of an intramolecular interaction important for the regulation of GIT1 functions. | Totaro, A., et al. 2007. Mol Biol Cell. 18: 5124-38. PMID: 17898078
- Decreased BMP2 signal in GIT1 knockout mice slows bone healing. | Sheu, TJ., et al. 2014. Mol Cell Biochem. 397: 67-74. PMID: 25138700
- GIT1 promotes lung cancer cell metastasis through modulating Rac1/Cdc42 activity and is associated with poor prognosis. | Chang, JS., et al. 2015. Oncotarget. 6: 36278-91. PMID: 26462147
- GIT1 is a novel prognostic biomarker and facilitates tumor progression via activating ERK/MMP9 signaling in hepatocellular carcinoma. | Chen, J., et al. 2015. Onco Targets Ther. 8: 3731-42. PMID: 26719701
- Alternative RNA splicing of the GIT1 gene is associated with neuroendocrine prostate cancer. | Lee, AR., et al. 2019. Cancer Sci. 110: 245-255. PMID: 30417466
- GIT1 is critical for formation of the CD31hiEmcnhi vessel subtype in coupling osteogenesis with angiogenesis via modulating preosteoclasts secretion of PDGF-BB. | Xu, T., et al. 2019. Bone. 122: 218-230. PMID: 30853660
- Neuroprotective Effect of Mangiferin against Parkinson's Disease through G-Protein-Coupled Receptor-Interacting Protein 1 (GIT1)-Mediated Antioxidant Defense. | Zhou, H., et al. 2023. ACS Chem Neurosci.. PMID: 37036451
- Dendronized chitosan hydrogel with GIT1 to accelerate bone defect repair through increasing local neovascular amount. | Cheng, L., et al. 2023. Bone Rep. 19: 101712. PMID: 37744736
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GIT1 Antibody (E-7) | sc-398637 | 200 µg/ml | $322.00 | |||
GIT1 Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-538484 | 200 µg Ab; 10 µg BP | $361.00 | |||
GIT1 Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-536108 | 200 µg Ab; 40 µg BP | $361.00 | |||
GIT1 Antibody (E-7): m-IgG2a BP-HRP Bundle | sc-548320 | 200 µg Ab; 10 µg BP | $361.00 |