γ-GCSm Antibody (G-4): sc-55585

γ-GCSm Antibody (G-4) is a mouse monoclonal IgG2b antibody that detects gamma-glutamylcysteine synthetase (γ-GCS) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). γ-GCS plays a crucial role as the rate-limiting enzyme in glutathione synthesis, a vital antioxidant that protects cells from oxidative stress and maintains cellular redox balance. Glutathione helps neutralize reactive oxygen species and supports various metabolic processes. γ-GCS protein consists of a heavy catalytic subunit and a light regulatory subunit, forming a heterodimeric zinc metalloprotein. This structure enables enzymatic activity, as the formation of a reversible disulfide bond between the subunits enhances function, allowing for a dynamic response to cellular stressors such as inflammation and oxidative damage. The human γ-GCS gene encoding the catalytic subunit is located on chromosome 6p12, while the regulatory subunit is found on chromosome 1p22-p21, highlighting the complexity of regulation and significance of expression in various physiological and pathological contexts. γ-GCS (G-4) antibody serves as an invaluable tool for researchers studying oxidative stress, cellular signaling, and antioxidant defense mechanisms.

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γ-GCSm Antibody (G-4) References:

1. Expression of heavy subunit of gamma-glutamylcysteine synthetase (gamma-GCSh) in human colorectal carcinoma.  |  Tatebe, S., et al. 2002. Int J Cancer. 97: 21-7. PMID: 11774239
2. Diminished immunoreactivity of gamma-glutamylcysteine synthetase in the airways of smokers' lung.  |  Harju, T., et al. 2002. Am J Respir Crit Care Med. 166: 754-9. PMID: 12204877
3. Gamma-glutamylcysteine synthetase and L-buthionine-(S,R)-sulfoximine: a new selection strategy for gene-transduced neural and hematopoietic stem/progenitor cells.  |  Lorico, A., et al. 2005. Hum Gene Ther. 16: 711-24. PMID: 15960602
4. Gamma-glutamylcysteine synthetase-based selection strategy for gene therapy of chronic granulomatous disease and graft-vs.-host disease.  |  Rappa, G., et al. 2007. Eur J Haematol. 78: 440-8. PMID: 17331133
5. Gamma-glutamylcysteine synthetase deficiency and hemolytic anemia.  |  Beutler, E., et al. 1990. Blood. 75: 271-3. PMID: 2294991
6. Overexpression of bacterial γ-glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar.  |  He, J., et al. 2015. New Phytol. 205: 240-54. PMID: 25229726
7. Enzymatic Production of Glutathione by Bifunctional γ-Glutamylcysteine Synthetase/Glutathione Synthetase Coupled with In Vitro Acetate Kinase-Based ATP Generation.  |  Jiang, Y., et al. 2016. Appl Biochem Biotechnol. 180: 1446-1455. PMID: 27380420
8. Inhibition of gamma-glutamylcysteine synthetase by L-methionine-S-sulfoximine.  |  Richman, PG., et al. 1973. J Biol Chem. 248: 6684-90. PMID: 4147652
9. gamma-Glutamylcysteine synthetase and GSH increase in quinone-induced oxidative stress in BPAEC.  |  Shi, MM., et al. 1994. Am J Physiol. 267: L414-21. PMID: 7943345
10. Expression and purification of human gamma-glutamylcysteine synthetase.  |  Misra, I. and Griffith, OW. 1998. Protein Expr Purif. 13: 268-76. PMID: 9675072

The preferred γ-GCSm antibody is γ-GCSm Antibody (E-4): sc-55586.