γ-GCSm Antibody (F-8): sc-166603

γ-GCSm Antibody (F-8) is a mouse monoclonal IgG2b antibody that detects gamma-glutamylcysteine synthetase (γ-GCS) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). γ-GCS plays a crucial role as the rate-limiting enzyme in glutathione synthesis, a vital antioxidant that protects cells from oxidative stress and maintains cellular redox balance. γ-GCS functions as a heterodimer composed of a heavy catalytic subunit and a light regulatory subunit, forming a zinc metalloprotein that responds to various cellular signals, including inflammation and oxidative stress. The catalytic subunit, encoded by a gene located on chromosome 6p12, consists of 367 amino acids and is essential for enzymatic activity, while the regulatory subunit, found on chromosome 1p22-p21, modulates enzyme function through reversible disulfide bond formation. This intricate regulation of γ-GCS activity maintains adequate glutathione levels, particularly in response to cellular stressors, making γ-GCS (F-8) antibody an invaluable tool for studying oxidative stress and related pathways in various biological contexts.

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γ-GCSm Antibody (F-8) References:

1. Melatonin induces gamma-glutamylcysteine synthetase mediated by activator protein-1 in human vascular endothelial cells.  |  Urata, Y., et al. 1999. Free Radic Biol Med. 27: 838-47. PMID: 10515588
2. Bleomycin upregulates expression of gamma-glutamylcysteine synthetase in pulmonary artery endothelial cells.  |  Day, RM., et al. 2002. Am J Physiol Lung Cell Mol Physiol. 282: L1349-57. PMID: 12003792
3. A spectrophotometric assay of gamma-glutamylcysteine synthetase and glutathione synthetase in crude extracts from tissues and cultured mammalian cells.  |  Volohonsky, G., et al. 2002. Chem Biol Interact. 140: 49-65. PMID: 12044560
4. Glutathione regulates the expression of gamma-glutamylcysteine synthetase via the Met4 transcription factor.  |  Wheeler, GL., et al. 2002. Mol Microbiol. 46: 545-56. PMID: 12406228
5. Structure-based identification of novel human gamma-glutamylcysteine synthetase inhibitors.  |  Hamilton, D., et al. 2007. Mol Pharmacol. 71: 1140-7. PMID: 17229871
6. Knock down of gamma-glutamylcysteine synthetase in rat causes acetaminophen-induced hepatotoxicity.  |  Akai, S., et al. 2007. J Biol Chem. 282: 23996-4003. PMID: 17573345
7. The gamma-glutamylcysteine synthetase gene of Leishmania is essential and involved in response to oxidants.  |  Mukherjee, A., et al. 2009. Mol Microbiol. 74: 914-27. PMID: 19818018
8. Electroacupuncture reduces oxidative stress response and improves secondary injury of intracerebral hemorrhage in rats by activating the peroxisome proliferator-activated receptor-γ/nuclear factor erythroid2-related factor 2/γ-glutamylcysteine synthetase pathway.  |  Luo, W., et al. 2024. Neuroreport. 35: 499-508. PMID: 38597270
9. Regulation of gamma-glutamylcysteine synthetase by protein phosphorylation.  |  Sun, WM., et al. 1996. Biochem J. 320 (Pt 1): 321-8. PMID: 8947504
10. Lipid peroxides, glutathione, gamma-glutamylcysteine synthetase and gamma-glutamyltranspeptidase activities in several tissues of rats following water-immersion stress.  |  Alptekin, N., et al. 1996. Pharmacol Res. 34: 167-9. PMID: 9051711

The preferred γ-GCSm antibody is γ-GCSm Antibody (E-4): sc-55586.