fzr Antibody (B-6): sc-166714

fzr Antibody (B-6) is a mouse monoclonal IgG1 antibody that detects fzr in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-fzr antibody (B-6) recognizes fzr, a member of the 7WD domain family, which plays a crucial role in cell cycle regulation by negatively regulating the levels of cyclins A, B, and B3. This regulation is vital for maintaining proper cell division and preventing uncontrolled proliferation, which can lead to tumorigenesis. Notably, fzr has been shown to suppress B-cell tumor formation, as retroviral overexpression of fzr in B-lymphoma cells significantly reduces tumor growth and enhances susceptibility to natural killer cell cytotoxicity. fzr′s ability to modulate cyclin levels is essential for ensuring that cells do not progress through additional division cycles, which can disrupt normal tissue homeostasis. Furthermore, loss of fzr function can lead to aberrant cell cycle progression, as seen in epidermal cells, where fzr causes an extra division cycle, and in salivary gland cells, where fzr inhibits endoreduplication. The implications of fzr′s regulatory functions extend to its potential as a therapeutic target in cancer treatment, making fzr monoclonal antibody (B-6) a valuable tool for researchers studying cell cycle dynamics and tumor biology.

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fzr Antibody (B-6) References:

1. Securin degradation is mediated by fzy and fzr, and is required for complete chromatid separation but not for cytokinesis.  |  Zur, A. and Brandeis, M. 2001. EMBO J. 20: 792-801. PMID: 11179223
2. The roles of Fzy/Cdc20 and Fzr/Cdh1 in regulating the destruction of cyclin B in space and time.  |  Raff, JW., et al. 2002. J Cell Biol. 157: 1139-49. PMID: 12082076
3. Mammalian Cdh1/Fzr mediates its own degradation.  |  Listovsky, T., et al. 2004. EMBO J. 23: 1619-26. PMID: 15029244
4. Terminal mitoses require negative regulation of Fzr/Cdh1 by Cyclin A, preventing premature degradation of mitotic cyclins and String/Cdc25.  |  Reber, A., et al. 2006. Development. 133: 3201-11. PMID: 16854973
5. Loss of the mammalian APC/C activator FZR1 shortens G1 and lengthens S phase but has little effect on exit from mitosis.  |  Sigl, R., et al. 2009. J Cell Sci. 122: 4208-17. PMID: 19861496
6. The APC/C activator FZR1 is essential for meiotic prophase I in mice.  |  Holt, JE., et al. 2014. Development. 141: 1354-65. PMID: 24553289
7. Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells.  |  The, I., et al. 2015. Nat Commun. 6: 5906. PMID: 25562820
8. APC/CFzr/Cdh1-Dependent Regulation of Planar Cell Polarity Establishment via Nek2 Kinase Acting on Dishevelled.  |  Weber, U. and Mlodzik, M. 2017. Dev Cell. 40: 53-66. PMID: 28041906
9. APC/CFzr regulates cardiac and myoblast cell numbers, and plays a crucial role during myoblast fusion.  |  Drechsler, M., et al. 2018. J Cell Sci. 131: PMID: 29898917
10. Revisiting degron motifs in human AURKA required for its targeting by APC/CFZR1.  |  Abdelbaki, A., et al. 2023. Life Sci Alliance. 6: PMID: 36450448

The preferred fzr antibody is fzr Antibody (DCS-266): sc-56312.