fzr Antibody (DCS-266): sc-56312

fzr Antibody (DCS-266) is a mouse monoclonal IgG1 kappa light chain antibody that detects fzr protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-fzr antibody (DCS-266) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. fzr protein, also known as Fizzy-related protein, plays a crucial role in cell cycle regulation and is a member of the 7WD domain family, which is conserved across eukaryotic species. fzr protein negatively regulates the levels of key mitotic cyclins, including cyclins A, B, and B3, thereby influencing cell cycle progression. Loss of fzr protein can lead to abnormal cell division, resulting in an extra division cycle in the epidermis and inhibiting endoreduplication in the salivary gland, which can significantly impact cellular growth and differentiation. Overexpression of fzr protein can disrupt normal mitotic processes, transforming mitotic cycles into endoreduplication cycles, which may contribute to tumorigenesis. Recent studies highlight fzr protein′s emerging role in enhancing B-lymphoma cells′ susceptibility to natural killer cell cytotoxicity, suggesting potential therapeutic applications in cancer treatment.

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fzr Antibody (DCS-266) References:

1. The mammalian Fizzy and Fizzy-related genes are regulated at the transcriptional and post-transcriptional levels.  |  Inbal, N., et al. 1999. FEBS Lett. 463: 350-4. PMID: 10606752
2. Phosphorylation of Cdc20/fizzy negatively regulates the mammalian cyclosome/APC in the mitotic checkpoint.  |  Yudkovsky, Y., et al. 2000. Biochem Biophys Res Commun. 271: 299-304. PMID: 10799291
3. Overexpression of murine fizzy-related (fzr) increases natural killer cell-mediated cell death and suppresses tumor growth.  |  Wang, CX., et al. 2000. Blood. 96: 259-63. PMID: 10891459
4. Securin degradation is mediated by fzy and fzr, and is required for complete chromatid separation but not for cytokinesis.  |  Zur, A. and Brandeis, M. 2001. EMBO J. 20: 792-801. PMID: 11179223
5. Timing of APC/C substrate degradation is determined by fzy/fzr specificity of destruction boxes.  |  Zur, A. and Brandeis, M. 2002. EMBO J. 21: 4500-10. PMID: 12198152
6. APC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timing.  |  Holt, JE., et al. 2012. Mol Biol Cell. 23: 3970-81. PMID: 22918942
7. The APC activator fizzy-related-1 (FZR1) is needed for preimplantation mouse embryo development.  |  Seah, MK., et al. 2012. J Cell Sci. 125: 6030-7. PMID: 23097041
8. PRL-3 Promotes Ubiquitination and Degradation of AURKA and Colorectal Cancer Progression via Dephosphorylation of FZR1.  |  Zhang, C., et al. 2019. Cancer Res. 79: 928-940. PMID: 30498084
9. Phosphorylation of the Anaphase Promoting Complex activator FZR1/CDH1 is required for Meiosis II entry in mouse male germ cell.  |  Tanno, N., et al. 2020. Sci Rep. 10: 10094. PMID: 32572094
10. FZR1 as a novel biomarker for breast cancer neoadjuvant chemotherapy prediction.  |  Liu, S., et al. 2020. Cell Death Dis. 11: 804. PMID: 32978372
11. LxCxD motif of the APC/C coactivator subunit FZR1 is critical for interaction with the retinoblastoma protein.  |  Ramanujan, A., et al. 2021. Exp Cell Res. 404: 112632. PMID: 33971196
12. Drosophila fizzy-related down-regulates mitotic cyclins and is required for cell proliferation arrest and entry into endocycles.  |  Sigrist, SJ. and Lehner, CF. 1997. Cell. 90: 671-81. PMID: 9288747

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-fzr Antibody (DCS-266) has 34 citations in a variety of scientific publications.

Primary antibodies like Anti-fzr Antibody (DCS-266) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

fzr Antibody (DCS-266) Specifications

Alternate Product Name: fzr

Product Clone Name: DCS-266

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: fzr

Epitope Species: human

Product Reactivity/Detection: fzr

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for fzr Antibody (DCS-266): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin