FIH-1 Antibody (D-12): sc-365016

FIH-1 Antibody (D-12) is a mouse monoclonal IgG1 antibody that detects Factor inhibiting HIF-1 (FIH-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-FIH-1 antibody (D-12) plays a crucial role in studying the regulation of hypoxia-inducible factor 1 alpha (HIF-1α) by functioning as an asparaginyl hydroxylase, specifically catalyzing the hydroxylation of asparagine residue 803 within the carboxy-terminal transactivation domain of HIF-1α. This post-translational modification is significant as FIH-1 (D-12) antibody helps reveal how HIF-1α interaction with essential co-activators is inhibited, thereby modulating the transcriptional activity of genes involved in the cellular response to hypoxia. Additionally, FIH-1 monoclonal antibody (D-12) aids in studying interactions with the von Hippel-Lindau (VHL) tumor suppressor protein, further contributing to the regulation of HIF-1α activity. FIH-1 (D-12) antibody demonstrates localization in the cytoplasm of transiently transfected human osteosarcoma cells, underscoring functional importance in cellular signaling pathways. The structural characteristics of FIH-1 include a jellyroll-like β-barrel that contains ferrous-binding triad residues, which are critical for enzymatic activity. The gene encoding human FIH-1 is located on chromosome 10q24, highlighting genetic significance in various biological processes.

The preferred FIH-1 antibody is FIH-1 Antibody (A-5): sc-271780.