Ferrochelatase Antibody (A-3): sc-377377

Ferrochelatase Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ferrochelatase of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Ferrochelatase (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ferrochelatase, also known as heme synthetase or protoheme ferro-lyase, plays a crucial role as the terminal enzyme in protoheme biosynthesis, catalyzing the insertion of ferrous iron into protoporphyrin IX, which is essential for the production of heme, a vital component of hemoglobin and various cytochromes. Defects in the FECH gene lead to erythropoietic protoporphyria (EPP), a dominantly inherited disorder characterized by photosensitivity and hepatobiliary complications. Ferrochelatase functions as a homodimeric protein associated with the mitochondrial membrane, featuring an N-terminal 54-amino acid transit peptide essential for mitochondrial localization. Nitric oxide-sensitive clusters within Ferrochelatase suggest a potential role in cellular signaling related to oxidative stress, highlighting Ferrochelatase′s importance in maintaining cellular homeostasis and function.

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Ferrochelatase Antibody (A-3) References:

1. Porphyrin-substrate binding to murine ferrochelatase: effect on the thermal stability of the enzyme.  |  Franco, R., et al. 2005. Biochem J. 386: 599-605. PMID: 15496139
2. Ferrochelatase consisting of wild-type and mutated subunits from patients with a dominant-inherited disease, erythropoietic protoporphyria, is an active but unstable dimer.  |  Ohgari, Y., et al. 2005. Hum Mol Genet. 14: 327-34. PMID: 15574461
3. Hepatic gene expression in protoporphyic Fech mice is associated with cholestatic injury but not a marked depletion of the heme regulatory pool.  |  Davies, R., et al. 2005. Am J Pathol. 166: 1041-53. PMID: 15793285
4. Production and characterization of erythropoietic protoporphyric heterodimeric ferrochelatases.  |  Najahi-Missaoui, W. and Dailey, HA. 2005. Blood. 106: 1098-104. PMID: 15831704
5. A point mutation affecting an SP1 binding site in the promoter of the ferrochelatase gene impairs gene transcription and causes erythropoietic protoporphyria.  |  Di Pierro, E., et al. 2005. Exp Hematol. 33: 584-91. PMID: 15850836
6. Photosystem II assembly in CP47 mutant of Synechocystis sp. PCC 6803 is dependent on the level of chlorophyll precursors regulated by ferrochelatase.  |  Sobotka, R., et al. 2005. J Biol Chem. 280: 31595-602. PMID: 16027152
7. Normal dermal ferrochelatase activity does not protect human skin from protoporphyrin-induced photosensitivity.  |  Elder, G. 2005. J Invest Dermatol. 125: 580; author reply 580-1. PMID: 16117803
8. Metallation of the transition-state inhibitor N-methyl mesoporphyrin by ferrochelatase: implications for the catalytic reaction mechanism.  |  Shipovskov, S., et al. 2005. J Mol Biol. 352: 1081-90. PMID: 16140324
9. Inhibition of ferrochelatase impairs vascular eNOS/NO and sGC/cGMP signaling.  |  Zhang, B., et al. 2018. PLoS One. 13: e0200307. PMID: 29985945
10. Ferrochelatase Deficiency Abrogated the Enhancement of Aminolevulinic Acid-mediated Protoporphyrin IX by Iron Chelator Deferoxamine.  |  Palasuberniam, P., et al. 2019. Photochem Photobiol. 95: 1052-1059. PMID: 30767226
11. Retinal Phenotyping of Ferrochelatase Mutant Mice Reveals Protoporphyrin Accumulation and Reduced Neovascular Response.  |  Sardar Pasha, SPB., et al. 2021. Invest Ophthalmol Vis Sci. 62: 36. PMID: 33620374
12. Photosensitivity and acute liver injury in myeloproliferative disorder secondary to late-onset protoporphyria caused by deletion of a ferrochelatase gene in hematopoietic cells.  |  Goodwin, RG., et al. 2006. Blood. 107: 60-2. PMID: 16150949

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ferrochelatase Antibody (A-3) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Ferrochelatase Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ferrochelatase Antibody (A-3) Specifications

Alternate Product Name: Ferrochelatase

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 329-369

Epitope Target: Ferrochelatase

Epitope Species: human

Product Reactivity/Detection: mitochondrial precursor and mature Ferrochelatase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Ferrochelatase Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin