Fc ε RIγ Antibody (F-1): sc-390221

Fc ε RIγ Antibody (F-1) is a mouse monoclonal IgG1 antibody that detects Fc ε RIγ in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Fc ε RIγ plays a crucial role in the immune response by serving as a signaling subunit of the high-affinity IgE receptor, which is essential for mediating allergic reactions. Fc ε RIγ is primarily located on the surface of mast cells and basophils, where Fc ε RIγ facilitates the binding of IgE antibodies to allergens, leading to cell activation and the subsequent release of inflammatory mediators such as histamines. The presence of Fc ε RIγ on these immune cells is vital for the initiation and amplification of hypersensitivity responses, making Fc ε RIγ a key player in allergic diseases. Structurally, Fc ε RIγ is composed of a single transmembrane domain and a cytoplasmic tail that contains immunoreceptor tyrosine activation motifs (ITAMs), which are critical for signal transduction. The phosphorylation of these ITAMs upon receptor activation is necessary for the downstream signaling cascades that result in the release of pro-inflammatory cytokines and other mediators, underscoring the importance of Fc ε RIγ in both normal immune function and pathological allergic responses.

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Fc ε RIγ Antibody (F-1) References:

1. Early TCR alpha beta expression promotes maturation of T cells expressing Fc epsilon RI gamma containing TCR/CD3 complexes.  |  Petersson, K. and Ivars, F. 2001. J Immunol. 166: 6616-24. PMID: 11359815
2. Amyloid-beta immunization effectively reduces amyloid deposition in FcRgamma-/- knock-out mice.  |  Das, P., et al. 2003. J Neurosci. 23: 8532-8. PMID: 13679422
3. Elf-1 binds to GGAA elements on the FcRgamma promoter and represses its expression.  |  Juang, YT., et al. 2007. J Immunol. 179: 4884-9. PMID: 17878388
4. Identification of human NK cells that are deficient for signaling adaptor FcRγ and specialized for antibody-dependent immune functions.  |  Hwang, I., et al. 2012. Int Immunol. 24: 793-802. PMID: 22962434
5. Pertussis toxin targets the innate immunity through DAP12, FcRγ, and MyD88 adaptor proteins.  |  Phongsisay, V., et al. 2017. Immunobiology. 222: 664-671. PMID: 28089363
6. Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8)/CLM-6 is an FcRγ-coupled receptor selectively expressed in mouse tissue plasmacytoid dendritic cells.  |  Kaitani, A., et al. 2018. Sci Rep. 8: 8259. PMID: 29844322
7. Distinct human circulating NKp30+FcεRIγ+CD8+ T cell population exhibiting high natural killer-like antitumor potential.  |  Correia, MP., et al. 2018. Proc Natl Acad Sci U S A. 115: E5980-E5989. PMID: 29895693
8. IL-18 Responsiveness Defines Limitations in Immune Help for Specialized FcRγ- NK Cells.  |  Anderko, RR., et al. 2020. J Immunol. 205: 3429-3442. PMID: 33188073
9. A tail of two ITAMs: GPVI/FcRγ and FcγRIIa's role in platelet activation and thrombus stability.  |  Neeves, KB. 2021. Res Pract Thromb Haemost. 5: e12564. PMID: 34263108
10. FcεRIγ-negative NK cells persist in vivo and enhance efficacy of therapeutic monoclonal antibodies in multiple myeloma.  |  Bigley, AB., et al. 2021. Blood Adv. 5: 3021-3031. PMID: 34357379

The preferred Fc ε RIγ antibody is Fc ε RIγ Antibody (E-12): sc-390222.