Fc ε RIγ Antibody (E-12): sc-390222

Fc ε RIγ Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Fc epsilon RI gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Fc ε RIγ (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Fc epsilon RI gamma protein plays a crucial role in immune response, particularly in mediating allergic reactions. Fc epsilon RI gamma exists as a tetrameric complex composed of a heavily glycosylated alpha chain, a beta chain, and two disulfide-linked gamma chains, with gamma chains being integral to the function of other Fc receptors. The presence of immunoreceptor tyrosine activation motif (ITAM) in cytoplasmic domains of beta and gamma subunits is essential for signal transduction, as phosphorylation of specific tyrosine residues within this motif is necessary for activation of mast cells and other hematopoietic cells. This signaling cascade is vital for release of mediators such as histamines, which are responsible for symptoms of allergies, and for secretion of lymphokines that drive hypersensitivity response. The ability of IgE Fc receptor to bind with high affinity to Fc region of immunoglobulin epsilon chains underscores Fc epsilon RI gamma′s importance in initiating and propagating allergic responses, making anti-Fc ε RIγ antibody (E-12) a valuable tool for research in immunology and allergy studies.

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Fc ε RIγ Antibody (E-12) References:

1. Mast cells, Fc epsilon RI, and IL-13 are required for development of airway hyperresponsiveness after aerosolized allergen exposure in the absence of adjuvant.  |  Taube, C., et al. 2004. J Immunol. 172: 6398-406. PMID: 15128831
2. Basophil CD63 expression assay on highly sensitized atopic donor leucocytes-a useful method in chronic autoimmune urticaria.  |  Gyimesi, E., et al. 2004. Br J Dermatol. 151: 388-96. PMID: 15327546
3. The gene and cDNA for the human high affinity immunoglobulin E receptor beta chain and expression of the complete human receptor.  |  Küster, H., et al. 1992. J Biol Chem. 267: 12782-7. PMID: 1535625
4. Identification of FcalphaRI as an inhibitory receptor that controls inflammation: dual role of FcRgamma ITAM.  |  Pasquier, B., et al. 2005. Immunity. 22: 31-42. PMID: 15664157
5. The human genes for the alpha and gamma subunits of the mast cell receptor for immunoglobulin E are located on human chromosome band 1q23.  |  Le Coniat, M., et al. 1990. Immunogenetics. 32: 183-6. PMID: 2146219
6. FcRγ-chain ITAM signaling is critically required for cross-presentation of soluble antibody-antigen complexes by dendritic cells.  |  Boross, P., et al. 2014. J Immunol. 193: 5506-14. PMID: 25355925
7. M. tuberculosis-Initiated Human Mannose Receptor Signaling Regulates Macrophage Recognition and Vesicle Trafficking by FcRγ-Chain, Grb2, and SHP-1.  |  Rajaram, MVS., et al. 2017. Cell Rep. 21: 126-140. PMID: 28978467
8. Human and rat mast cell high-affinity immunoglobulin E receptors: characterization of putative alpha-chain gene products.  |  Shimizu, A., et al. 1988. Proc Natl Acad Sci U S A. 85: 1907-11. PMID: 2964640
9. TC21/RRas2 regulates glycoprotein VI-FcRγ-mediated platelet activation and thrombus stability.  |  Janapati, S., et al. 2018. J Thromb Haemost.. PMID: 29883056
10. [Foreign body as cause of a large urethral calculus and diverticulum formation].  |  Hackel, W. 1968. Z Urol Nephrol. 61: 827-9. PMID: 4978125
11. Temporal activation of nontransmembrane protein-tyrosine kinases following mast cell Fc epsilon RI engagement.  |  Penhallow, RC., et al. 1995. J Biol Chem. 270: 23362-5. PMID: 7559493
12. Characterization of the gene for the human high affinity IgE receptor (Fc epsilon RI) alpha-chain.  |  Pang, J., et al. 1993. J Immunol. 151: 6166-74. PMID: 8245459

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fc ε RIγ Antibody (E-12) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Fc ε RIγ Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fc ε RIγ Antibody (E-12) Specifications

Alternate Product Name: Fc ε RIγ

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-86

Epitope Target: Fc RIγ

Epitope Species: human

Product Reactivity/Detection: Fc ε RIγ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Fc ε RIγ Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin