FBL20 Antibody (D-4): sc-515274
- FBL20 Antibody (D-4) is a mouse monoclonal IgG2b κ FBL20 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 66-81 within an internal region of FBL20 of human origin
- FBL20 Antibody (D-4) is recommended for detection of FBL20 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-FBL20 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for FBL20 Antibody (D-4) for WB applications. These reagents are now offered in bundles with FBL20 Antibody (D-4) (see ordering information below).
FBL20 Antibody (D-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects FBL20 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FBL20 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBL20, also known as F-box/LRR-repeat protein 20, is a 436 amino acid protein encoded by the human gene FBXL20 and is characterized by the presence of a 40-amino acid F-box region and twelve leucine-rich repeats. This unique structure enables FBL20 to function as a key component of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex, which plays a vital role in substrate recognition and recruitment for ubiquitination. FBL20′s ability to target specific proteins for degradation, such as cyclins and β-catenin, is essential for regulating various cellular processes, including cell cycle, immune response, and developmental pathways. FBL20 exists in two isoforms due to alternative splicing, which may contribute to functional diversity in different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FBL20 Antibody (D-4) References:
- Identification of a family of human F-box proteins. | Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
- A family of mammalian F-box proteins. | Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
- The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. | Craig, KL. and Tyers, M. 1999. Prog Biophys Mol Biol. 72: 299-328. PMID: 10581972
- cDNA cloning and expression analysis of new members of the mammalian F-box protein family. | Ilyin, GP., et al. 2000. Genomics. 67: 40-7. PMID: 10945468
- Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. | Schulman, BA., et al. 2000. Nature. 408: 381-6. PMID: 11099048
- A new subfamily of structurally related human F-box proteins. | Ilyin, GP., et al. 2002. Gene. 296: 11-20. PMID: 12383498
- FBXL20 acts as an invasion inducer and mediates E-cadherin in colorectal adenocarcinoma. | Zhu, J., et al. 2014. Oncol Lett. 7: 2185-2191. PMID: 24932313
- F-box/LRR-repeat protein 7 is genetically associated with Alzheimer's disease. | Tosto, G., et al. 2015. Ann Clin Transl Neurol. 2: 810-20. PMID: 26339675
- Lipopolysaccharide modulates p300 and Sirt1 to promote PRMT1 stability via an SCFFbxl17-recognized acetyldegron. | Lai, Y., et al. 2017. J Cell Sci. 130: 3578-3587. PMID: 28883095
- F-Box Proteins and Cancer. | Yumimoto, K., et al. 2020. Cancers (Basel). 12: PMID: 32429232
- FBXL20-mediated ubiquitination triggers the proteasomal degradation of 4-1BB. | Sun, R. and Lim, SO. 2022. FEBS J. 289: 4549-4563. PMID: 35112462
- The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro. | Winston, JT., et al. 1999. Genes Dev. 13: 270-83. PMID: 9990852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBL20 Antibody (D-4) | sc-515274 | 200 µg/ml | $316.00 | |||
FBL20 Antibody (D-4): m-IgG Fc BP-HRP Bundle | sc-531327 | 200 µg Ab; 10 µg BP | $354.00 | |||
FBL20 Antibody (D-4): m-IgGκ BP-HRP Bundle | sc-524990 | 200 µg Ab, 40 µg BP | $354.00 | |||
FBL20 Antibody (D-4): m-IgG2b BP-HRP Bundle | sc-550277 | 200 µg Ab; 10 µg BP | $354.00 | |||
FBL20 Antibody (D-4) AC | sc-515274 AC | 500 µg/ml, 25% agarose | $416.00 | |||
FBL20 Antibody (D-4) HRP | sc-515274 HRP | 200 µg/ml | $316.00 | |||
FBL20 Antibody (D-4) FITC | sc-515274 FITC | 200 µg/ml | $330.00 | |||
FBL20 Antibody (D-4) PE | sc-515274 PE | 200 µg/ml | $343.00 | |||
FBL20 Antibody (D-4) Alexa Fluor® 488 | sc-515274 AF488 | 200 µg/ml | $357.00 | |||
FBL20 Antibody (D-4) Alexa Fluor® 546 | sc-515274 AF546 | 200 µg/ml | $357.00 | |||
FBL20 Antibody (D-4) Alexa Fluor® 594 | sc-515274 AF594 | 200 µg/ml | $357.00 | |||
FBL20 Antibody (D-4) Alexa Fluor® 647 | sc-515274 AF647 | 200 µg/ml | $357.00 | |||
FBL20 Antibody (D-4) Alexa Fluor® 680 | sc-515274 AF680 | 200 µg/ml | $357.00 | |||
FBL20 Antibody (D-4) Alexa Fluor® 790 | sc-515274 AF790 | 200 µg/ml | $357.00 | |||
FBL20 (D-4) Neutralizing Peptide | sc-515274 P | 100 µg/0.5 ml | $68.00 |