Fatty Acid Synthase Antibody (G-11) recommended for detection of Fatty Acid Synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Fatty Acid Synthase Antibody (G-11): sc-48357

Fatty Acid Synthase Antibody (G-11) is rated 4.7 out of 5 by 29.
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Datasheets

    • Anti-Fatty Acid Synthase Antibody (G-11) is a mouse monoclonal IgG1 (kappa light chain) Fatty Acid Synthase antibody provided at 200 µg/ml
    • raised against amino acids 2205-2504 of Fatty Acid Synthase of human origin
    • Anti-Fatty Acid Synthase Antibody (G-11) is recommended for detection of Fatty Acid Synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Fatty Acid Synthase Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Fatty Acid Synthase (G-11): sc-48357.

Can Fatty Acid Synthase (G-11): sc-48357 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Fatty Acid Synthase (G-11): sc-48357 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2018-09-18
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Rated 5 out of 5 by from Excellent It is very good for western blotting in muscle and liver tissue and the dilution is 1:2000.
Date published: 2019-10-12
Rated 4 out of 5 by from works well for WB This antibody works well for immunoblotting liver lysates.
Date published: 2019-05-07
Rated 5 out of 5 by from great WB in C2C12 WB: 1:1000 Cells:C2C12 Very good: Clear and specific band.
Date published: 2019-01-25
Rated 5 out of 5 by from Excellent! Really clean signal in skeletal muscle and adipose tissue lysates.
Date published: 2018-07-24
Rated 4 out of 5 by from A Good Quality antibody for FAS We are fully satisfied with the quality of this antibody in detecting fatty acid synthase in a MIA PaCa-2 pancreatic cancer cell line as well as mouse pancreas.
Date published: 2018-01-05
Rated 5 out of 5 by from it's perfect We made an experiment with Fatty Acid Synthase Antibody and found it very useful.  It provides clear,obtained specific band and specific results.
Date published: 2017-10-18
Rated 5 out of 5 by from As goog as expected I used this antibody at 1:500 with MEF cell lysates and my transfer time was 1 hour. The band looks very good for FAS at the expected size.
Date published: 2017-09-21
Rated 5 out of 5 by from FASN antibody on mouse eyelid paraffin secitons This antibody worked very well for immunostaining of paraffin sections of mouse eyelid tissues
Date published: 2017-08-30
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