Fatty Acid Synthase Antibody (A-5): sc-55580

Fatty Acid Synthase Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Fatty Acid Synthase (FAS) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-FAS antibody (A-5) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. FAS plays a crucial role in lipid metabolism, specifically in the biosynthesis of fatty acids, which are essential for cellular structure and function. FAS catalyzes the conversion of acetyl-CoA and malonyl-CoA into long-chain saturated fatty acids, such as palmitate, utilizing NADPH as a cofactor. This process is vital for energy storage and membrane formation, and is particularly important in rapidly dividing cells, such as those found in tumors, where fatty acid synthesis is often upregulated to support cell growth and survival. The human FAS gene encodes a 2,504 amino acid protein that functions as a homodimer, and FAS mRNA is variably expressed, with high levels found in tissues such as the brain, lung, and liver. Understanding FAS expression and regulation is critical, as dysregulation is associated with various metabolic disorders and cancers, making FAS a significant target for therapeutic intervention.

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Fatty Acid Synthase Antibody (A-5) References:

1. Fatty acid synthase: an early molecular marker of progression of prostatic adenocarcinoma to androgen independence.  |  Myers, RB., et al. 2001. J Urol. 165: 1027-32. PMID: 11176534
2. Increased fatty acid synthase is a therapeutic target in mesothelioma.  |  Gabrielson, EW., et al. 2001. Clin Cancer Res. 7: 153-7. PMID: 11205903
3. Pharmacological inhibition of fatty acid synthase activity produces both cytostatic and cytotoxic effects modulated by p53.  |  Li, JN., et al. 2001. Cancer Res. 61: 1493-9. PMID: 11245456
4. Human fatty acid synthase: role of interdomain in the formation of catalytically active synthase dimer.  |  Chirala, SS., et al. 2001. Proc Natl Acad Sci U S A. 98: 3104-8. PMID: 11248039
5. Increased fatty acid synthase as a therapeutic target in androgen-independent prostate cancer progression.  |  Pizer, ES., et al. 2001. Prostate. 47: 102-10. PMID: 11340632
6. Fatty acid synthase (FASN) regulates the mitochondrial priming of cancer cells.  |  Schroeder, B., et al. 2021. Cell Death Dis. 12: 977. PMID: 34675185
7. Association between fatty acid synthase and adipophilin expression in triple-negative breast cancer.  |  Yoshikawa, K., et al. 2022. Mol Clin Oncol. 16: 80. PMID: 35251631
8. Statin therapy inhibits fatty acid synthase via dynamic protein modifications.  |  Trub, AG., et al. 2022. Nat Commun. 13: 2542. PMID: 35538051
9. Fatty acid synthase (FASN) signalome: A molecular guide for precision oncology.  |  Menendez, JA., et al. 2024. Mol Oncol. 18: 479-516. PMID: 38158755
10. Human fatty acid synthase: properties and molecular cloning.  |  Jayakumar, A., et al. 1995. Proc Natl Acad Sci U S A. 92: 8695-9. PMID: 7567999
11. Isolation and chromosomal mapping of genomic clones encoding the human fatty acid synthase gene.  |  Jayakumar, A., et al. 1994. Genomics. 23: 420-4. PMID: 7835891
12. The animal fatty acid synthase: one gene, one polypeptide, seven enzymes.  |  Smith, S. 1994. FASEB J. 8: 1248-59. PMID: 8001737

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fatty Acid Synthase Antibody (A-5) has 107 citations in a variety of scientific publications.

Primary antibodies like Anti-Fatty Acid Synthase Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fatty Acid Synthase Antibody (A-5) Specifications

Alternate Product Name: Fatty Acid Synthase

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2205-2504

Epitope Target: Fatty Acid Synthase

Epitope Species: human

Product Reactivity/Detection: Fatty Acid Synthase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 107 citations in various scientific publications

Available Conjugates/Forms for Fatty Acid Synthase Antibody (A-5): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790

The preferred Fatty Acid Synthase antibody is Fatty Acid Synthase Antibody (G-11): sc-48357.