ETHE1 Antibody (B-12): sc-393869

ETHE1 Antibody (B-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects ETHE1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ETHE1 antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ETHE1, also known as ethylmalonic encephalopathy 1 or hepatoma subtracted clone one protein, consists of 254 amino acids and belongs to the metallo-beta-lactamase superfamily and glyoxalase II family. ETHE1 is primarily localized in the cytoplasm, nucleus, and mitochondrial matrix, which is crucial for sulfide catabolism, a process that helps detoxify harmful sulfide compounds in cells. ETHE1 binds two zinc ions per subunit, which is essential for structural integrity and enzymatic function. Additionally, ETHE1 interacts directly with RELA protein, inhibiting RELA translocation to the nucleus and thereby suppressing p53-induced apoptosis, a critical pathway in cancer biology. The gene encoding ETHE1 is located on human chromosome 19q13.31, and mutations in this gene are associated with ethylmalonic encephalopathy, a severe metabolic disorder that manifests in infancy with symptoms such as high levels of ethylmalonic acid, neurodevelopmental delays, recurrent petechiae, acrocyanosis, and a high mortality rate within the first decade of life.

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ETHE1 Antibody (B-12) References:

1. A novel protein overexpressed in hepatoma accelerates export of NF-kappa B from the nucleus and inhibits p53-dependent apoptosis.  |  Higashitsuji, H., et al. 2002. Cancer Cell. 2: 335-46. PMID: 12398897
2. Identification of a gene causing human cytochrome c oxidase deficiency by integrative genomics.  |  Mootha, VK., et al. 2003. Proc Natl Acad Sci U S A. 100: 605-10. PMID: 12529507
3. Ethylmalonic encephalopathy is caused by mutations in ETHE1, a gene encoding a mitochondrial matrix protein.  |  Tiranti, V., et al. 2004. Am J Hum Genet. 74: 239-52. PMID: 14732903
4. Enhanced deacetylation of p53 by the anti-apoptotic protein HSCO in association with histone deacetylase 1.  |  Higashitsuji, H., et al. 2007. J Biol Chem. 282: 13716-25. PMID: 17353187
5. Identification of new mutations in the ETHE1 gene in a cohort of 14 patients presenting with ethylmalonic encephalopathy.  |  Mineri, R., et al. 2008. J Med Genet. 45: 473-8. PMID: 18593870
6. Loss of ETHE1, a mitochondrial dioxygenase, causes fatal sulfide toxicity in ethylmalonic encephalopathy.  |  Tiranti, V., et al. 2009. Nat Med. 15: 200-5. PMID: 19136963
7. Ethylmalonic encephalopathy: application of improved biochemical and molecular diagnostic approaches.  |  Drousiotou, A., et al. 2011. Clin Genet. 79: 385-90. PMID: 20528888
8. Combined treatment with oral metronidazole and N-acetylcysteine is effective in ethylmalonic encephalopathy.  |  Viscomi, C., et al. 2010. Nat Med. 16: 869-71. PMID: 20657580
9. Multiple sources of metabolic disturbance in ETHE1-related ethylmalonic encephalopathy.  |  Barth, M., et al. 2010. J Inherit Metab Dis. 33 Suppl 3: S443-53. PMID: 20978941
10. Ethylmalonic encephalopathy. Another patient from Kuwait.  |  Ismail, EA., et al. 2009. Neurosciences (Riyadh). 14: 78-80. PMID: 21048580
11. Proteomics reveals that redox regulation is disrupted in patients with ethylmalonic encephalopathy.  |  Palmfeldt, J., et al. 2011. J Proteome Res. 10: 2389-96. PMID: 21410200
12. Novel ETHE1 mutation in a carrier couple having prior offspring affected with ethylmalonic encephalopathy: Genetic analysis, clinical management and reproductive outcome.  |  Walsh, DJ., et al. 2010. Mol Med Rep. 3: 223-6. PMID: 21472225

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ETHE1 Antibody (B-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ETHE1 Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ETHE1 Antibody (B-12) Specifications

Alternate Product Name: ETHE1

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 185-214

Epitope Target: ETHE1

Epitope Species: human

Product Reactivity/Detection: ETHE1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ETHE1 Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin