ERp57 Antibody (TO-2) recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

ERp57 Antibody (TO-2): sc-80648

ERp57 Antibody (TO-2) is rated 5.0 out of 5 by 3.
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Datasheets

    • ERp57 Antibody (TO-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a synthetic peptide corresponding to amino acids 484-502 of ERp57 of human origin
    • recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

llo, I need secondery antibody for(ERp57 Antibody (TO-2)) to be used in mouse.

Asked by: bettys
Thank you for your question. You can find our recommended antibodies for this primary antibody here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-06-13

Can ERp57 (TO-2): sc-80648 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, ERp57 (TO-2): sc-80648 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Positive band observed in WB in human ERp57 Positive band observed in WB in human ERp57 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-06-15
Rated 5 out of 5 by from Clean Clean, crisp signal in WB in mouse ERp57 transfected 293T and Daudi whole cell lysates. -SCBT QC
Date published: 2015-01-22
Rated 5 out of 5 by from Strong Strong, clear band obtained in WB in in HeLa and whole cell lysate.-SCBT QC
Date published: 2013-05-20
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